To Study the Anti-cancer Effects of Shigella Flexneri in AspC-1 Pancreatic Cancer Cell Line in Approach to Bax and bcl-2 Genes.

Journal of Gastrointestinal Cancer
Amir KhodavirdipourRana Zarean

Abstract

Cancer is the uncontrolled division of cells and can be caused by genetic or environmental factors. Pancreatic cancer is one of the deadliest among all cancers. The role of bacteria as an anticancer agent dates back to almost 100 years ago. The microbiome has recently become a focus of research in carcinogenesis and even pancreatic cancer. Shigella flexneri is a gram-negative bacterium, which causes shigellosis with symptoms such as diarrhea, fever, and stomach cramps in human. Shigella flexneri may play a very important role in the internal pathways of apoptosis and may induce apoptosis in some of the cancerous cells. In this experiment bacteria were cultured on Salmonella-Shigella agar, then inoculated into BHI Broth medium. After sonication, the protein concentration of the bacterium was measured by using the ZellBio Sensitive Protein Bradford Assay kit. MTT assay was performed to obtain IC50 for the said bacterial protein. Later by cDNA kit synthesized the cDNA based on the RNA template. In the end, the results were analyzed using real-time PCR and the expression of Bax and Bcl-2 genes was measured before and after treatment. The results showed that Shigella flexneri has the potential anti-proliferative effect in pancreatic...Continue Reading

References

Oct 15, 2010·Nature Reviews. Cancer·Neil S Forbes
Nov 16, 2013·Nature Reviews. Immunology·Richard S HotchkissDidier Payen
Dec 17, 2015·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Lindsey A TorreAhmedin Jemal
Feb 3, 2016·Lancet·Terumi KamisawaKyoichi Takaori
Apr 10, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yağmur KirazYusuf Baran
Dec 13, 2016·International Journal of Molecular Sciences·Atul Ranjan, Tomoo Iwakuma
Mar 21, 2017·Current Opinion in Microbiology·Anja FriedrichAnja Lührmann
Jul 26, 2017·Caspian Journal of Internal Medicine·Versa Omrani-NavaiFatima Toghani
May 18, 2018·Nature·Qiao Zhou, Douglas A Melton
Aug 30, 2018·Nature Communications·Song WuYusuf A Hannun

❮ Previous
Next ❯

Citations

Jan 5, 2021·Journal of Gastrointestinal Cancer·Amir KhodavirdipourMohammad Yousef Alikhani
Feb 20, 2021·Clinics and Research in Hepatology and Gastroenterology·Annemilaï Tijeras-RaballandCindy Neuzillet

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Related Papers

The Journal of Pathology and Bacteriology
A E FRANCIS
Trudy Leningradskogo instituta ėpidemiologii, mikrobiologii, i gigieny im. Pastera
V A ARBUZOVA
Postȩpy higieny i medycyny doświadczalnej
I Kétyi
Acta Microbiologica Academiae Scientiarum Hungaricae
K RaussT Angyal
Archivum Immunologiae Et Therapiae Experimentalis
S SlopekM Mulczyk
© 2022 Meta ULC. All rights reserved