Tobacco mosaic virus (TMV) and potato virus X (PVX) coat proteins confer heterologous interference to PVX and TMV infection, respectively

The Journal of General Virology
A A BazziniR N Beachy

Abstract

Replication of Potato virus X (PVX) was reduced in transgenic protoplasts that accumulated wild-type coat protein (CPWT) of Tobacco mosaic virus (TMV) or a mutant CP, CP(T42W), that produced highly ordered states of aggregation, including pseudovirions. This reaction is referred to as heterologous CP-mediated resistance. However, protoplasts expressing a CP mutant that abolished aggregation and did not produce pseudovirions, CPT28W, did not reduce PVX replication. Similarly, in transgenic tobacco plants producing TMV CPWT or CP(T42W), there was a delay in local cell-to-cell spread of PVX infection that was not observed in CP(T28W) plants or in non-transgenic plants. The results suggest that the quaternary structure of the TMV CP regulates the mechanism(s) of heterologous CP-mediated resistance. Similarly, transgenic protoplasts that produced PVX CP conferred transient protection against infection by TMV RNA. Transgenic plants that accumulated PVX CP reduced the cell-to-cell spread of infection and resulted in a delay in systemic infection following inoculation with TMV or TMV RNA. Heterologous CP-mediated resistance was characterized by a brief delay in systemic infection, whilst homologous CP-mediated resistance conferred redu...Continue Reading

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Citations

Jul 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·A A BazziniS Asurmendi
Dec 9, 2014·Frontiers in Plant Science·Valérie Nicaise
Feb 15, 2017·Molecular Biotechnology·Amir HameedShahid Mansoor

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