PMID: 8602170Mar 26, 1996Paper

Tobacco plants expressing T4 endonuclease V show enhanced sensitivity to ultraviolet light and DNA alkylating agents

Mutation Research
G LapointeD H Evans

Abstract

DNA repair processes and UV-filtering pigments protect organisms from the cytotoxicity of UV light and endow plants with a high degree of natural UV resistance. In an attempt to further enhance this UV resistance we have constructed transgenic tobacco lines that express a DNA repair enzyme encoded by the bacteriophage T4 denV gene. The denV gene encodes endonuclease V, an enzyme which initiates base excision repair of cyclobutane pyrimidine dimers. Its presence is expected to provide transgenotes with a repair pathway complementary to, but likely distinct from, the repair pathways found in tobacco. The denV gene, flanked by a CaMV 35S promoter and poly(A) addition site, was introduced into tobacco and mature plants regenerated. The transgenotes expressed high levels of a UV-specific endonuclease and no such activity was found in control plants. Curiously, assays which detected several different biological endpoints showed that the denV+ transgenotes were also hypersensitive to UV-C light. This hypersensitivity segregated with the denV gene and was not caused by altered concentrations of UV-filtering pigments. Moreover, the denV+ transgenotes were also hypersensitive to high levels of baseless lesions that would be generated by ...Continue Reading

References

Sep 1, 1989·Mutation Research·M L Dodson, R S Lloyd
Aug 25, 1989·Nucleic Acids Research·D MattanovichH Katinger
Oct 25, 1989·Nucleic Acids Research·W J Shen, B G Forde
Jan 1, 1988·Annual Review of Biochemistry·T LindahlY Nakabeppu
Jan 1, 1988·Annual Review of Biochemistry·A Sancar, G B Sancar
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D VetterP D Sadowski
Oct 1, 1993·The Plant Journal : for Cell and Molecular Biology·A Batschauer
Dec 1, 1989·Trends in Ecology & Evolution·M M CaldwellM Tevini

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Biosynthetic Transformations

Biosyntheic transformtions are multi-step, enzyme-catalyzed processes where substrates are converted into more complex products in living organisms. Simple compounds are modified, converted into other compounds, or joined together to form macromolecules. Discover the latest research on biosynthetic transformations here.