Tobacco smoke and nicotine suppress expression of activating signaling molecules in human dendritic cells

Toxicology Letters
Nuha AlkhattabiLucy C Fairclough

Abstract

Cigarette smoke has significant toxic effects on the immune system, and increases the risk of developing autoimmune diseases; one immunosuppressive effect of cigarette smoke is that it inhibits the T cell-stimulating, immunogenic properties of myeloid dendritic cells (DCs). As the functions of DCs are regulated by intra-cellular signaling pathways, we investigated the effects of cigarette smoke extract (CSE) and nicotine on multiple signaling molecules and other regulatory proteins in human DCs to elucidate the molecular basis of the inhibition of DC maturation and function by CSE and nicotine. Maturation of monocyte-derived DCs was induced with the TLR3-agonist poly I:C or with the TLR4-agonist lipopolysaccharide, in the absence or presence of CSE or nicotine. Reverse-phase protein microarray was used to quantify multiple signaling molecules and other proteins in cell lysates. Particularly in poly I:C-matured DCs, cigarette smoke constituents and nicotine suppressed the expression of signaling molecules associated with DC maturation and T cell stimulation, cell survival and cell migration. In conclusion, constituents of tobacco smoke suppress the immunogenic potential of DCs at the signaling pathway level.

Citations

Jun 7, 2019·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·G DaeschleinM Jünger
Jun 3, 2021·International Journal of Molecular Sciences·Yu SawadaMotonobu Nakamura
Jan 3, 2021·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Federico BarberaFrancesco Klinger
Mar 3, 2020·Chemical Research in Toxicology·Pei-Hsuan ChuDavid L Gerhold

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