Tobamovirus infection is independent of HSP101 mRNA induction and protein expression

Virus Research
Tyrell CarrSteven A Whitham

Abstract

Heat shock protein 101 (HSP101) has been implicated in tobamovirus infections by virtue of its ability to enhance translation of mRNAs possessing the 5'Omega-leader of Tobacco mosaic virus (TMV). Enhanced translation is mediated by HSP101 binding to a CAA-repeat motif in TMV Omega leader. CAA repeat sequences are present in the 5' leaders of other tobamoviruses including Oilseed rape mosaic virus (ORMV), which infects Arabidopsis thaliana. HSP101 is one of eight HSP100 gene family members encoded by the A. thaliana genome, and of these, HSP101 and HSP98.7 are predicted to encode proteins localized to the cytoplasm where they could potentially interact with TMV RNA. Analysis of the expression of the HSP100s showed that only HSP101 mRNA transcripts were induced significantly by ORMV in A. thaliana. The induction of HSP101 mRNA was also correlated with an increase in its protein levels and was independent of defense-related signaling pathways involving salicylic acid, jasmonic acid, or ethylene. A. thaliana mutants lacking HSP101, HSP98.7, or both supported wild-type levels of ORMV replication and movement. Similar results were obtained for TMV infection in Nicotiana benthamiana plants silenced for HSP101, demonstrating that HSP10...Continue Reading

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Citations

Nov 1, 2006·Molecular Plant-microbe Interactions : MPMI·Steven A WhithamMichael M Goodin
Apr 13, 2007·Molecular Plant-microbe Interactions : MPMI·Chunling YangSteven A Whitham
Sep 3, 2010·Plant Molecular Biology·Amanjot Singh, Anil Grover
May 28, 2011·Molecular Biotechnology·T D Lodha, J Basak
May 8, 2007·Annual Review of Phytopathology·Roger P WiseSteven A Whitham
Jul 1, 2015·Critical Reviews in Biotechnology·Ratnesh Chandra Mishra, Anil Grover
Jul 20, 2007·The Plant Journal : for Cell and Molecular Biology·Shanfa LuVincent L Chiang
Feb 17, 2015·Virus Research·Tetsuya ChujoMasayuki Ishikawa
Jul 17, 2007·Nature Reviews. Microbiology·Ian BrierleyRobert J C Gilbert
Jun 5, 2012·Molecular BioSystems·Jackson R MoellerSteven A Whitham

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