Tocopherol induced angiogenesis in placental vascular network in late pregnant ewes.

Reproductive Biology and Endocrinology : RB&E
Ramanathan K KasimanickamCraig D Thatcher

Abstract

Tocopherols have biphasic, proangiogenic and antiangiogenic therapeutic effects. The objective of this clinical trial was to clarify tocopherol's placental angiogenic potential in late pregnant ewes following oral supplementation. Eighteen pregnant ewes during late gestation were selected for this study. Ewes were given oral supplementation of 500 mg of alpha-tocopherol (aT; N=6) or 1000 mg of gamma-tocopherol (gT; N=7) or placebo (CON; N=5) once daily from 107 to 137 days post breeding. Serum was obtained at weekly intervals and tissue samples were obtained at the end of supplementation to: 1) evaluate tocopherol concentrations in serum, uterus and placentome; 2) evaluate relative mRNA expressions of Vascular Endothelial Growth Factor (VEGF), Placental Growth Factor (PlGF), endothelial Nitric Oxide Synthase (eNOS) and Hypoxia Inducible Factors (HIF) in uterus, caruncle and cotyledon; 3) analyze the morphometry of the placental vascular network. Supplementation of aT or gT resulted in increased concentrations in serum, placentome and uterus compared to control (P<0.05). In aT group, mRNA expressions of PlGF, eNOS and HIF-1alpha in cotyledon were greater than the CON group. In gT group, mRNA expressions of VEGF, eNOS, HIF-1 alph...Continue Reading

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Citations

May 25, 2011·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Leucio D Vieira-FilhoAna D O Paixão
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Dec 18, 2018·IUBMB Life·Jean-Marc Zingg
Aug 20, 2019·Molecular and Cellular Biochemistry·Vaishali KastureSadhana Joshi

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Datasets Mentioned

BETA
AF071015

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

FarcLac
ImageJ
SAS
SAS System
FracLac

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