Toehold-mediated nonenzymatic amplification circuit on graphene oxide fluorescence switching platform for sensitive and homogeneous microRNA detection

Analytica Chimica Acta
Ru HuangDa Xing

Abstract

A novel graphene oxide (GO) fluorescence switch-based homogenous system has been developed to solve two problems that are commonly encountered in conventional GO-based biosensors. First, with the assistance of toehold-mediated nonenzymatic amplification (TMNA), the sensitivity of this system greatly surpasses that of previously described GO-based biosensors, which are always limited to the nM range due to the lack of efficient signal amplification. Second, without enzymatic participation in amplification, the unreliability of detection resulting from nonspecific desorption of DNA probes on the GO surface by enzymatic protein can be avoided. Moreover, the interaction mechanism of the double-stranded TMNA products contains several single-stranded toeholds at two ends and GO has also been explored with combinations of atomic force microscopy imaging, zeta potential detection, and fluorescence assays. It has been shown that the hybrids can be anchored to the surface of GO through the end with more unpaired bases, and that the other end, which has weaker interaction with GO, can escape GO adsorption due to the robustness of the central dsDNA structures. We verified this GO fluorescence switch-based detection system by detecting micr...Continue Reading

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Citations

Dec 15, 2015·Analytical Chemistry·Richard M Graybill, Ryan C Bailey
Mar 22, 2018·The Analyst·Yongqiang ChengZhengping Li
Sep 16, 2018·Analytical and Bioanalytical Chemistry·Zhenping LiuZhihua Tao
Jan 21, 2017·Analytical Chemistry·Jungho KimDal-Hee Min

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