ToF-SIMS study of differentiation of human bone-derived stromal cells: new insights into osteoporosis

Analytical and Bioanalytical Chemistry
Kaija SchaepeJürgen Janek

Abstract

Lipids have numerous important functions in the human body, as they form the cells' plasma membranes and play a key role in many disease states, presumably also in osteoporosis. Here, the fatty acid composition of the outer plasma membranes of cells differentiated into the osteogenic and adipogenic direction is studied with surface-sensitive time-of-flight secondary ion mass spectrometry (ToF-SIMS). For data evaluation, principal component analysis (PCA) is applied. Human (bone-derived) mesenchymal stromal cells (hMSCs) from an osteoporotic donor and a control donor are compared to reveal differences in the fatty acid composition of the membranes. The chemical information is correlated to staining and real-time quantitative polymerase chain reaction (rt-qPCR) results to provide insight into the gene expression of several differentiation markers on the RNA level. Adipogenic differentiation of hMSCs from a non-osteoporotic donor correlates with increased relative intensities of all fatty acids under investigation. After osteogenic differentiation of non-osteoporotic cells, the relative mass signal intensities of unsaturated fatty acids such as oleic and linoleic acids are increased. However, the osteoporotic cells show increased ...Continue Reading

References

Mar 11, 2000·Biomaterials·K Anselme
Jul 30, 2003·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M Riminucci, P Bianco
Jan 25, 2005·Bone·Sabine WenischReinhard Schnettler
Jul 30, 2005·Nature Reviews. Drug Discovery·Markus R Wenk
Dec 2, 2005·Nature·Frederick R Maxfield, Ira Tabas
Aug 26, 2006·Nature Clinical Practice. Rheumatology·Clifford J Rosen, Mary L Bouxsein
Sep 6, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Lan-Juan ZhaoHong-Wen Deng
Jun 6, 2008·Current Opinion in Rheumatology·Gustavo Duque
Dec 26, 2008·Food & Nutrition Research·Helena Lindmark Månsson
Dec 15, 2010·Cell·Markus R Wenk
Feb 11, 2011·Analytical and Bioanalytical Chemistry·J A FernándezR Rodríguez-Puertas
May 25, 2012·The FEBS Journal·Claudio R Santos, Almut Schulze
Aug 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Suwimon BoonrungsimanMolly M Stevens
Jun 5, 2013·Molecular Nutrition & Food Research·Sharon A Murphy, Anna Nicolaou
Aug 21, 2013·Current Opinion in Chemical Biology·Melissa K Passarelli, Andrew G Ewing
Nov 26, 2013·Biomaterials·Kristina GlenskeSabine Wenisch
Mar 7, 2014·Current Osteoporosis Reports·Sandra BermeoGustavo Duque
Mar 22, 2014·Annals of the New York Academy of Sciences·Erica L Scheller, Clifford J Rosen
Apr 8, 2014·Joint, Bone, Spine : Revue Du Rhumatisme·Pierre HardouinBernard Cortet
Apr 16, 2014·The Lancet. Diabetes & Endocrinology·Maureen J Devlin, Clifford J Rosen
Jun 24, 2014·Archives of Biochemistry and Biophysics·Beata Lecka-Czernik, Lance A Stechschulte
Dec 2, 2015·Therapeutic Advances in Endocrinology and Metabolism·Emanuela A GrecoSilvia Migliaccio
Jan 27, 2016·Biointerphases·Kaija SchaepeJürgen Janek
Sep 28, 2016·Lipids in Health and Disease·Marc Pilon
Nov 5, 2016·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Alena-Svenja WagnerSabine Wenisch
Mar 4, 2017·Biointerphases·Vytis ČižinauskasVitalis Briedis

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Citations

May 23, 2020·Biointerphases·Christine KernMarcus Rohnke
Dec 27, 2019·World Journal of Stem Cells·Antonio Casado-DíazJosé Manuel Quesada-Gómez

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