PMID: 597036Jan 1, 1977

Togavirus RNA: reversible effect of urea on genomes and absence of subgenomic viral RNA in Kunjin virus-infected cells

Archives of Virology
R W Boulton, E G Westaway

Abstract

Electrophoretic analyses showed that no RNase-sensitive RNA smaller than the genome was specified by the flavivirus Kunjin in infected Vero cells during the period of maximum RNA and protein synthesis. In contrast, RNA extracted from Sindbis virus-infected cells under similar conditions included the expected 42S RNA (equivalent to the genome) and the smaller 26S (interjacent) RNA. Treatment of the genome of both togaviruses with 12 M urea produced a reversible (possibly conformational) change; measurement of the molecular weights of the treated RNAs by co-electrophoresis with fully denatured ribosomal RNA markers in SDS-polyacrylamide gels yielded a value of 2.1 X 10(6) if 8 M urea was incorporated in the gels and 4.2 X 10(6) if urea was omitted from the gels. These results indicate that flavivirus messenger RNA is represented solely by the intact genome of m.wt. 4.2 X 10(6).

References

Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·N GlanvilleA E Smith
Oct 26, 1973·Biochimica Et Biophysica Acta·L ReijndersP Borst
May 15, 1974·Journal of Molecular Biology·J L SaborioG Koch
Jan 1, 1974·Cold Spring Harbor Symposia on Quantitative Biology·M T HsuN Davidson
Mar 1, 1974·The Journal of General Virology·J C Clegg, S I Kennedy
Jul 1, 1971·Japanese Journal of Microbiology·C Nishimura, H Tsukeda
Jan 1, 1972·Journal of Virology·B M Arif, P Faulkner
Apr 1, 1969·Journal of Virology·D W TrentA A Qureshi

Citations

Jan 1, 1983·Archives of Virology·M L NgE G Westaway
Jan 1, 1985·Archives of Virology·S Ozden, B Poirier
Aug 15, 2015·Methods : a Companion to Methods in Enzymology·Radhakrishnan PadmanabhanKyung H Choi
May 26, 2012·PLoS Neglected Tropical Diseases·Cheng HuangSlobodan Paessler
Jun 19, 2019·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Kenneth HodgeSarin Chimnaronk

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Arboviruses
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