PMID: 6988275Jan 1, 1980Paper

Tolbutamide stimulation and inhibition of insulin release: studies of the underlying ionic mechanisms in isolated rat islets

Diabetologia
J C Henquin

Abstract

The effects of tolbutamide on insulin release, 45Ca2+ uptake and 86Rb+ efflux were studied in isolated rat islets. At a low glucose concentration (75 mg/dl), tolbutamide (20-500 microgram/ml) produced a rapid, dose-dependent increase in insulin release from perifused islets. After 30-40 min however, the rate of secretion as well as the potentiating effect of theophylline were inversely related to the concentration of sulphonylurea. The monophasic release of insulin triggered by tolbutamide (100 microgram/ml) at low glucose could be evoked again by removing and reintroducing the drug, or by temporarily withdrawing calcium or adding cobalt to the medium. Tolbutamide (20 microgram/ml) accelerated and potentiated the biphasic insulin release in response to a secondary stimulation by glucose (150 mg/dl). By contrast, 100 microgram/ml tolbutamide reduced the releasing effect of glucose to a slow increase in secretion rates. Theophylline normalized the second phase of release, but did not restore the rapid phase. Tolbutamide stimulated 45Ca2+ influx (2 min-uptake) in islet cells; this effect was maximum immediately after addition of the drug and decreased later on, exhibiting a monophasic pattern. Glucose stimulation of Ca2+ uptake (5...Continue Reading

References

Jul 25, 1977·Biochemical and Biophysical Research Communications·J C Henquin
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Sep 1, 1976·The American Journal of Physiology·J C Henquin, A E Lambert
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Jun 1, 1975·The American Journal of Physiology·J C Henquin, A E Lambert
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Citations

Dec 16, 1980·Molecular and Cellular Biochemistry·A Sener, W J Malaisse
Oct 1, 1985·Naunyn-Schmiedeberg's Archives of Pharmacology·L NorlundJ Sehlin
Jul 1, 1980·Naunyn-Schmiedeberg's Archives of Pharmacology·S KawazuW J Malaisse
Dec 1, 1988·Pflügers Archiv : European journal of physiology·M Bozem, J C Henquin
Nov 1, 1986·Pflügers Archiv : European journal of physiology·G TrubeT Ohno-Shosaku
Jul 1, 1981·Diabetologia·I B Täljedal
Jul 31, 1985·European Journal of Pharmacology·F VetterleinG Schmidt
May 27, 1986·European Journal of Pharmacology·M G GarrinoJ C Henquin
Jan 1, 1989·Pharmacology & Therapeutics·C J BaileyV Marks
Dec 15, 1992·Biochimica Et Biophysica Acta·S J Ashcroft, F M Ashcroft
May 28, 1990·Molecular and Cellular Endocrinology·M A WahlH P Ammon
Dec 1, 1992·Molecular and Cellular Endocrinology·M A WahlH P Ammon
Dec 1, 1994·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·S Saha, B Hellman
Mar 1, 2000·British Journal of Pharmacology·G PatanèF Purrello
Feb 1, 1993·European Journal of Clinical Investigation·B H Wolffenbuttel, T W Van Haeften
Jan 1, 1992·Fundamental & Clinical Pharmacology·P Petit, M M Loubatières-Mariani
Mar 1, 1984·Acta Physiologica Scandinavica·L Norlund, J Sehlin
Apr 1, 1990·The Journal of Pharmacy and Pharmacology·E J VerspohlM Mark
Nov 15, 1986·British Medical Journal·P LudmanG F Joplin

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