Toll-Like Receptor 2 and Lipoprotein-Like Lipoproteins Enhance Staphylococcus aureus Invasion in Epithelial Cells

Infection and Immunity
Minh-Thu NguyenFriedrich Götz

Abstract

Staphylococcus aureus contains a certain subclass of lipoproteins, the so-called lipoprotein-like lipoproteins (Lpl's), that not only represent Toll-like receptor 2 (TLR2) ligands but are also involved in host cell invasion. Here we addressed the question of which factors contribute to Lpl-mediated invasion of epithelial cells and keratinocytes. For this purpose, we compared the invasiveness of USA300 and its Δlpl mutant under different conditions. In the presence of the matrix proteins IgG, fibrinogen (Fg), and fibronectin (Fn), and of fetal bovine serum (FBS), the invasion ratio was increased in both strains, and always more in USA300 than in its Δlpl mutant. Interestingly, when we compared the invasion of HEK-0 and HEK-TLR2 cells, the cells expressing TLR2 showed a 9-times-higher invasion frequency. When HEK-TLR2 cells were additionally stimulated with a synthetic lipopeptide, Pam3CSK4 (P3C), the invasion frequency was further increased. A potential reason for the positive effect of TLR2 on invasion could be that TLR2 activation by P3C also activates F-actin formation. Here we show that S. aureus invasion depends on a number of factors, on the host side as well as on the bacterial side.

References

Oct 1, 1985·Infection and Immunity·S K OgawaF D Lowy
Aug 26, 1998·The New England Journal of Medicine·F D Lowy
Nov 24, 1998·Infection and Immunity·B E Menzies, I Kourteva
May 27, 1999·Microbial Pathogenesis·J K EllingtonM C Hudson
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·D ZhouJ E Galán
Dec 31, 2003·The Journal of Experimental Medicine·Sean E DoyleGenhong Cheng
May 19, 2004·Archives of Dermatological Research·Andor PivarcsiLajos Kemény
Dec 6, 2005·International Journal of Medical Microbiology : IJMM·Christian KohlerMichael Hecker
Oct 10, 2006·Annals of Biomedical Engineering·Elena B Lomakina, Richard E Waugh
Sep 29, 2009·International Journal of Medical Microbiology : IJMM·Bhanu Sinha, Martin Fraunholz
Nov 6, 2009·International Journal of Medical Microbiology : IJMM·John A Wright, Sean P Nair
Jul 10, 2010·Journal of Leukocyte Biology·Marco A Quevedo-DiazAndrei E Medvedev
Jan 22, 2011·Journal of Thrombosis and Haemostasis : JTH·A AssingerI Volf
Mar 19, 2013·The Journal of Biological Chemistry·Prexy ShahJonathan P Butchar
Jan 22, 2014·International Journal of Medical Microbiology : IJMM·Friedrich GötzThilo Stehle
May 16, 2014·BioMed Research International·Nayeli Alva-MurilloAlejandra Ochoa-Zarzosa
May 27, 2015·Molecular Microbiology·Patrick EbnerFriedrich Götz
Jul 8, 2015·Current Genetics·Patrick EbnerFriedrich Götz
Oct 28, 2015·Infection and Immunity·Minh Thu NguyenFriedrich Götz
Jul 10, 2016·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M StrobelS Niemann
Aug 12, 2016·Microbiology and Molecular Biology Reviews : MMBR·Minh Thu Nguyen, Friedrich Götz

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Citations

Sep 14, 2019·Cellular Microbiology·Paula M TribelliFriedrich Götz
Jul 23, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jessica S HookJessica G Moreland

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