Toll-like receptor 2 enhances ZO-1-associated intestinal epithelial barrier integrity via protein kinase C

Gastroenterology
Elke CarioD K Podolsky

Abstract

Protein kinase C (PKC) has been implicated in regulation of intestinal epithelial integrity in response to lumenal bacteria. Intestinal epithelial cells (IECs) constitutively express Toll-like receptor (TLR)2, which contains multiple potential PKC binding sites. The aim of this study was to determine whether TLR2 may activate PKC in response to specific ligands, thus potentially modulating barrier function in IECs. TLR2 agonist (synthetic bacterial lipopeptide Pam(3)CysSK4, peptidoglycan)-induced activation of PKC-related signaling cascades were assessed by immunoprecipitation, Western blotting, immunofluorescence, and kinase assays-combined with functional transfection studies in the human model IEC lines HT-29 and Caco-2. Transepithelial electrical resistance characterized intestinal epithelial barrier function. Stimulation with TLR2 ligands led to activation (phosphorylation, enzymatic activity, translocation) of specific PKC isoforms (PKCalpha and PKCdelta). Phosphorylation of PKC by TLR2 ligands was blocked specifically by transfection with a TLR2 deletion mutant. Ligand-induced activation of TLR2 greatly enhanced transepithelial resistance in IECs, which was prevented by pretreatment with PKC-selective antagonists. This e...Continue Reading

References

Dec 1, 1995·The Journal of Biological Chemistry·A C Newton
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·R O Stuart, S K Nigam
Feb 28, 1994·Biochemical and Biophysical Research Communications·M GschwendtF Marks
Feb 1, 1994·Trends in Biochemical Sciences·L V Dekker, P J Parker
Oct 1, 1993·The Journal of Cell Biology·M S BaldaJ M Anderson
Mar 27, 1999·European Journal of Biochemistry·A KnightN Fairweather
Sep 14, 1999·The American Journal of Physiology·J R TurnerJ L Madara
Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·E CarioD K Podolsky
Jun 2, 2000·Gastroenterology·T KinugasaH C Reinecker
Jul 6, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M HirschfeldJ J Weis
Dec 29, 2000·Nature·X XuX Wang
Apr 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Y W HsuW W Lin
Jul 10, 2001·American Journal of Physiology. Cell Physiology·J C SongJ B Matthews
Aug 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A T GewirtzJ L Madara
Aug 30, 2001·Nature Immunology·T HorngR Medzhitov
Nov 7, 2001·The Journal of Experimental Medicine·A CastrilloL Boscá
Jan 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yonca BulutMoshe Arditi
Feb 28, 2002·Annual Review of Immunology·Charles A Janeway, Ruslan Medzhitov
Mar 20, 2002·Molecular Pharmacology·Marcelo G Kazanietz
Mar 30, 2002·The Journal of Biological Chemistry·Maria T AbreuMoshe Arditi
May 3, 2002·The Journal of Biological Chemistry·Daisuke IwakiYoshio Kuroki
Aug 9, 2002·The New England Journal of Medicine·Daniel K Podolsky
Oct 10, 2002·American Journal of Physiology. Cell Physiology·Jaekyung Cecilia SongJeffrey B Matthews
Nov 6, 2002·Journal of Biochemistry·Yasuhito Shirai, Naoaki Saito
Nov 6, 2002·Journal of Biochemistry·Shigeru Nakashima
Nov 15, 2002·European Journal of Immunology·Michael MorrPeter F Mühlradt

❮ Previous
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Citations

Aug 31, 2010·Inflammatory Bowel Diseases·Elke Cario
Jul 17, 2009·The British Journal of Nutrition·P C CalderJ Zhao
May 28, 2008·Immunology and Cell Biology·Xian-Ming ChenNicholas F LaRusso
Nov 6, 2009·Mucosal Immunology·E C LavelleE M Creagh
Mar 22, 2013·Mucosal Immunology·M Fukata, M Arditi
Nov 3, 2005·Nature Clinical Practice. Gastroenterology & Hepatology·Alessio Fasano, Terez Shea-Donohue
Dec 5, 2008·Nature Reviews. Cancer·Seth Rakoff-Nahoum, Ruslan Medzhitov
Feb 7, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Cécile BeaurepaireDerek M McKay
Jan 21, 2011·Current Opinion in Gastroenterology·Rebeca SantaolallaMaria T Abreu
Jan 14, 2012·Current Opinion in Gastroenterology·Rebeca Santaolalla, Maria T Abreu
Jul 20, 2010·Journal of Pediatric Gastroenterology and Nutrition·Reed A DimmittRobin G Lorenz
Aug 14, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ludmila KhailovaBohuslav Dvorak
Jun 18, 2010·American Journal of Physiology. Renal Physiology·David W GoodBruns A Watts
Nov 17, 2007·The Journal of Clinical Investigation·Matam Vijay-KumarAndrew T Gewirtz
May 9, 2012·The Journal of Clinical Investigation·Camille JungFrederick Barreau
Jul 13, 2013·BMC Gastroenterology·Jing ChengReetta Satokari
Oct 26, 2011·Microbial Cell Factories·Jerry M Wells
Sep 21, 2012·Journal of the International Society of Sports Nutrition·Manfred LamprechtJoachim F Greilberger
Aug 15, 2013·Gut Pathogens·Loris R LopetusoAntonio Gasbarrini
Aug 30, 2008·PloS One·Zaruhi A KhachatryanRustam I Aminov
Dec 29, 2010·International Journal of Inflammation·Markus Schuppler, Martin J Loessner
Apr 16, 2013·BioMed Research International·Javier Rivera GuzmanChristian Jobin
Jun 3, 2014·International Journal of Molecular Sciences·Marianna Parlato, Garabet Yeretssian
Feb 5, 2013·The Journal of Allergy and Clinical Immunology·I-Hsin KuoLisa A Beck

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