Toll-like receptor 2 mediates the induction of IL-10 in corneal fibroblasts in response to Fusarium solu

Immunology and Cell Biology
Hui GuoJie Zhao

Abstract

Toll-like receptors (TLRs) are key components of the innate immune system that detect microbial infection and trigger antimicrobial host responses. To determine the role of TLR2 in the expression of pro- and anti-inflammatory cytokines in corneal fibroblasts challenged by fungi, we used siRNA specific for TLR2 to knockdown TLR2 expression in telomerase-immortalized human stroma fibroblasts (THSF). TLR2 expression was assessed by immunocytochemistry, reverse transcription (RT)-PCR and western blotting analyses, and we found that THSF transfected with TLR2-siRNA plasmid exhibited a reduced level of TLR2 when compared with the control cells transfected with empty plasmid. Then the transfected and control cells were stimulated by hyphae or conidia of Fusarium solu and mRNA levels of IL-1beta and IL-10 were measured by real-time RT-PCR. We observed that stimulation of control cells with F. solu resulted in elevation of IL-1beta and IL-10 mRNA with hyphae being more stimulatory than conidia. In contrast, F. solu-stimulated IL-10 production by transfected THSF was severely impaired (with a decrease of approximately 82% in hyphae and 70% in conidia stimulation), while IL-1beta production was partially inhibited (with a reduction of 60 ...Continue Reading

References

Jul 31, 2001·The Journal of Biological Chemistry·F Re, J L Strominger
Feb 28, 2002·Annual Review of Immunology·Charles A Janeway, Ruslan Medzhitov
May 7, 2002·The Journal of Infectious Diseases·Mihai G NeteaBart Jan Kullberg
Aug 13, 2002·The Journal of Biological Chemistry·Salamatu S MambulaStuart M Levitz
Oct 23, 2002·The Journal of Experimental Medicine·Andreas SingJürgen Heesemann
Jan 14, 2003·Annual Review of Immunology·Kiyoshi TakedaShizuo Akira
Mar 26, 2003·Journal of Molecular Biology·Dag R SørensenMouldy Sioud
Apr 30, 2003·The Journal of Experimental Medicine·Benjamin N GantnerDavid M Underhill
Jun 7, 2003·Science·Gregory M Barton, Ruslan Medzhitov
Feb 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vanessa RedeckeEyal Raz
Feb 26, 2004·The European Respiratory Journal·R Bals, P S Hiemstra
Mar 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mihai G NeteaBart Jan Kullberg
Jul 2, 2004·Nature Reviews. Immunology·Shizuo Akira, Kiyoshi Takeda
Jan 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Susan L UprichardFrancis V Chisari
Feb 3, 2005·Medical Mycology·Alexander RoederHans Christian Korting
Feb 21, 2006·The British Journal of Ophthalmology·S Rodriguez-MartinezJ C Cancino-Diaz
Aug 29, 2006·Ophthalmology·Lixin XieShiying Sun
Nov 14, 2006·Eye & Contact Lens·Sandhya A IyerRyan C Wagoner
Nov 15, 2006·Current Pharmaceutical Design·Mihai G NeteaBart Jan Kullberg
May 15, 2007·Current Opinion in Infectious Diseases·Philip Aloysius Thomas, Pitchairaj Geraldine

❮ Previous
Next ❯

Citations

Jan 15, 2013·Burns : Journal of the International Society for Burn Injuries·Adam G Peres, Joaquín Madrenas
Dec 7, 2010·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L G WoodP G Gibson
May 29, 2009·Innate Immunity· Jie ZhaoFu-Shin X Yu
Aug 5, 2014·Evolutionary Bioinformatics Online·Maria NeedhamsenMeghan G Thomas
Dec 26, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hong-Zhen YangZhuo-Wei Hu
Jan 1, 2015·3 Biotech·Amandeep KaurVirender Kumar
Oct 28, 2017·Journal of Immunology Research·Dong-Dong WuXin-Ying Ji

❮ Previous
Next ❯

Related Concepts

Related Feeds