Toll-like receptor 4 activation on human amniotic epithelial cells is a risk factor for pregnancy loss

Journal of Research in Medical Sciences : the Official Journal of Isfahan University of Medical Sciences
Hossein MotedayyenRamezan Ali Taheri

Abstract

Maternal-fetal tolerance plays a fundamental role in the maintenance of pregnancy. However, this immunological tolerance can be influenced by intrauterine infections. Human amniotic epithelial cells (hAECs) have immunomodulatory effects and respond to invading pathogens through expressing various toll-like receptors (TLRs). We hypothesize that bacteria or bacterial products affect the immunosuppressive effects of hAECs through TLR stimulation. Here, we investigated how a successful pregnancy can be threatened by TLR4 activation on hAECs on lipopolysaccharide (LPS) engagement. hAECs were isolated from the amniotic membrane received from six healthy pregnant women. The immunophenotyping of hAECs was studied by flow cytometry. The isolated hAECs (4 × 105 cells/ml) were cultured in 24-well plates in the presence or absence of LPS (5 μg/ml). After 24, 48, and 72 h of incubation, the culture supernatants of hAECs were collected, and the levels of interleukin-5 (IL-5), IL-6, IL-1β, tumor necrosis factor-alpha (TNF-α), transforming growth factor-beta 1 (TGF-β1), and prostaglandin E2 (PGE2) were measured by enzyme-linked immunosorbent assay. TLR4 activation showed a stimulatory effect on TGF-β1 production of hAECs (P < 0.001-0.05). PGE2...Continue Reading

Citations

Apr 18, 2020·Prosthetics and Orthotics International·Cody L McDonaldSara J Morgan
Jul 7, 2020·Frontiers in Physiology·Justin G PadronClaire E Kendal-Wright

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Methods Mentioned

BETA
flow cytometry
enzyme-linked immunoasorbent assay
enzyme-linked immunosorbent assay

Software Mentioned

GraphPad
FlowJo
GraphPad Prism

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