PMID: 20654065Jul 27, 2010Paper

Toll-like receptor 4 expression in macrophages in endotoxin-induced uveitis in Wistar rats

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology
Wei ChenHong Lu

Abstract

To investigate the dynamics and distribution of toll-like receptor 4 (TLR4) in uvea-resident tissue macrophages during endotoxin-induced uveitis (EIU) in Wistar rats. Fifty Wistar rats were randomly divided into five groups (n = 10 per group) based on the following time points: before LPS injection (0 h, control group) and 6, 12, 24, and 48 h after LPS injection. All the rats (except the control group) received a footpad injection of 200 microg of vibrio cholera lipopolysaccharide (LPS). The intensity of anterior segment inflammation was evaluated after the LPS injection. Ten rats each were killed before LPS injection and 6, 12, 24, 48 h after injection. The iris-ciliary body complex and choroid from each eye were removed and cut into segments. Immunohistochemical localization of TLR4 and a resident tissue macrophage marker, cluster of differentiation 163 (CD163), was performed on whole mount isolated iris-ciliary body complexes and choroids. TLR4+ and CD163+ cells in the iris were counted, and the cell density (cells/mm(2)) was calculated. For CD163+ cells, the percent of round pleiomorphic cells in positive staining cells was calculated. The distribution patterns and the phenotypes of cells expressing these two proteins were ...Continue Reading

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