Abstract
Pericytes are multifunctional, polymorphic perivascular cells that lie within the microvessel basal lamina, are located on the abluminal side of endothelial cells, and are thought to play a regulatory role in capillary leak observed in sepsis. Toll-Like receptor 4 (TLR-4) has been implicated as the proximal transmembrane receptor for the LPS/CD 14 complex during the activation of lipopolysacharide (LPS)-induced sepsis. It is our hypothesis that TLR-4 is present on lung pericytes and is up-regulated in response to LPS. Rat microvascular lung pericytes were isolated and cultured. Cells from passage 3-5 were used and treated with LPS (control, 10 ng/mL, and 100 ng/mL) for 18 h. Immunostaining and immunoblotting were performed to detect the presence of CD-14, TLR-2, and TLR-4. Real-time polymerase chain reaction was used to analyze the presence and quantity of mRNA for CD-14, TLR-2, and TLR-4. Immunostaining and immunoblotting revealed the presence of CD-14, TLR-2, and TLR-4 in pericytes from each treatment group, and real-time polymerase chain reaction confirmed the presence of mRNA for CD-14, TLR-2, and TLR-4. An increase in the mRNA was observed in CD-14, TLR-2, and TLR-4 in the presence of increasing LPS 4 h after treatment. At...Continue Reading
References
May 1, 1991·Genes & Development·D S SchneiderK V Anderson
May 30, 1991·Nature·N J Gay, F J Keith
Oct 1, 1990·Transplantation·J S Pober, R S Cotran
Jan 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·F L RockJ F Bazan
Dec 8, 1998·The Journal of Experimental Medicine·C J KirschningM Rothe
Apr 10, 1999·The Journal of Biological Chemistry·J C ChowF Gusovsky
May 21, 1999·Science·J A HoffmannR A Ezekowitz
Aug 3, 1999·The Journal of Clinical Investigation·S FrantzR A Kelly
Sep 2, 1999·Critical Care Medicine·S M Opal, J Cohen
Feb 7, 2001·The Journal of Biological Chemistry·E FaureM Arditi
May 19, 2000·Cytokine & Growth Factor Reviews·T K MeansM J Fenton
Jul 13, 2000·Biochemical and Biophysical Research Communications·P L WangK Ohura
Oct 7, 2000·Clinical and Experimental Pharmacology & Physiology·D E Sims
Oct 29, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·T MatsumuraK Onozaki
Apr 26, 2001·The Journal of Surgical Research·C J DenteJ G Tyburski
May 8, 2001·Cells, Tissues, Organs·G Allt, J G Lawrenson
Feb 20, 2003·Nature Medicine·Jie Fan, Asrar B Malik
Feb 21, 2003·The New England Journal of Medicine·Margaret S HerridgeUNKNOWN Canadian Critical Care Trials Group
Mar 27, 2004·American Journal of Respiratory Cell and Molecular Biology·Lynne ArmstrongAnn B Millar
Apr 10, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Katherine A BarsnessRobert C McIntyre
Dec 23, 2004·Shock·Hironori TsujimotoHidetaka Mochizuki
Mar 2, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Gregory J BrunnJeffrey L Platt
May 6, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Lutz HamannRalf R Schumann
May 11, 2005·Kidney International·Didier DuclouxPierre Tiberghien
Sep 21, 2005·American Journal of Respiratory Cell and Molecular Biology·Michael A Matthay, Guy A Zimmerman
Citations
Nov 7, 2008·Cellular and Molecular Neurobiology·Tsuyoshi NishiokuYasufumi Kataoka
May 17, 2014·PloS One·Ludmila KhailovaPaul E Wischmeyer
May 19, 2007·The Journal of Surgical Research·David A EdelmanChristopher P Steffes
May 22, 2007·American Journal of Surgery·David A EdelmanChristopher P Steffes
Aug 26, 2006·The Journal of Surgical Research·David A EdelmanChristopher Steffes
Jun 21, 2014·Clinical Hemorheology and Microcirculation·Qingbin WuRuijuan Xiu
Jan 5, 2011·Respiratory Physiology & Neurobiology·Ricardo FernándezPaula P Cortes
Jan 21, 2016·Carcinogenesis·Hridayesh PrakashLiudmila Umansky
Apr 23, 2017·Brain, Behavior, and Immunity·Ádám Nyúl-TóthIstván A Krizbai
Mar 13, 2014·Pulmonary Circulation·Irshad AliElizabeth R Jacobs
Jan 12, 2007·The Journal of Trauma·David A EdelmanChristopher P Steffes
Feb 19, 2008·Shock·Hironori TsujimotoHidetaka Mochizuki
Apr 11, 2013·Anesthesiology·Ludmila KhailovaPaul E Wischmeyer
May 21, 2009·European Journal of Clinical Investigation·B KollerD Hartl
Jun 3, 2016·Current Opinion in Pulmonary Medicine·Justin M OldhamFernando J Martinez
Oct 25, 2017·Advances in Wound Care·Lin Chen, Luisa A DiPietro
Jun 18, 2019·Mediators of Inflammation·Paulina RudziakPaulina M Kowalewska
Dec 7, 2013·The Journal of Biological Chemistry·Irene Guijarro-MuñozLaura Sanz
Jun 21, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anamika BoseSubrata Majumdar
Nov 22, 2016·Frontiers in Immunology·Rocío NavarroLaura Sanz