Toll-like receptor-4 message is up-regulated in lipopolysaccharide-exposed rat lung pericytes

The Journal of Surgical Research
David A EdelmanChristopher P Steffes

Abstract

Pericytes are multifunctional, polymorphic perivascular cells that lie within the microvessel basal lamina, are located on the abluminal side of endothelial cells, and are thought to play a regulatory role in capillary leak observed in sepsis. Toll-Like receptor 4 (TLR-4) has been implicated as the proximal transmembrane receptor for the LPS/CD 14 complex during the activation of lipopolysacharide (LPS)-induced sepsis. It is our hypothesis that TLR-4 is present on lung pericytes and is up-regulated in response to LPS. Rat microvascular lung pericytes were isolated and cultured. Cells from passage 3-5 were used and treated with LPS (control, 10 ng/mL, and 100 ng/mL) for 18 h. Immunostaining and immunoblotting were performed to detect the presence of CD-14, TLR-2, and TLR-4. Real-time polymerase chain reaction was used to analyze the presence and quantity of mRNA for CD-14, TLR-2, and TLR-4. Immunostaining and immunoblotting revealed the presence of CD-14, TLR-2, and TLR-4 in pericytes from each treatment group, and real-time polymerase chain reaction confirmed the presence of mRNA for CD-14, TLR-2, and TLR-4. An increase in the mRNA was observed in CD-14, TLR-2, and TLR-4 in the presence of increasing LPS 4 h after treatment. At...Continue Reading

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Citations

May 19, 2007·The Journal of Surgical Research·David A EdelmanChristopher P Steffes
May 22, 2007·American Journal of Surgery·David A EdelmanChristopher P Steffes
Aug 26, 2006·The Journal of Surgical Research·David A EdelmanChristopher Steffes
Jun 21, 2014·Clinical Hemorheology and Microcirculation·Qingbin WuRuijuan Xiu
Jan 5, 2011·Respiratory Physiology & Neurobiology·Ricardo FernándezPaula P Cortes
Apr 23, 2017·Brain, Behavior, and Immunity·Ádám Nyúl-TóthIstván A Krizbai
Jan 12, 2007·The Journal of Trauma·David A EdelmanChristopher P Steffes
Feb 19, 2008·Shock·Hironori TsujimotoHidetaka Mochizuki
May 21, 2009·European Journal of Clinical Investigation·B KollerD Hartl
Jun 3, 2016·Current Opinion in Pulmonary Medicine·Justin M OldhamFernando J Martinez
Oct 25, 2017·Advances in Wound Care·Lin Chen, Luisa A DiPietro
Jun 18, 2019·Mediators of Inflammation·Paulina RudziakPaulina M Kowalewska
Jun 21, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anamika BoseSubrata Majumdar
Nov 22, 2016·Frontiers in Immunology·Rocío NavarroLaura Sanz

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