Toll-like receptor 4 promotes fibrosis in bleomycin-induced lung injury in mice

Genetics and Molecular Research : GMR
X X LiH P Dai

Abstract

The specific role of Toll-like receptor 4 (TLR4) in bleomycin-induced lung fibrosis of mice, a model of human idiopathic pulmonary fibrosis, has not been characterized. We injected bleomycin intratracheally into TLR4 knockout (TLR4(-/-)) and wild-type (WT) mice. Twenty-one days after injection, mice were sacrificed and their lungs were harvested for pathological, hydroxyproline, mRNA expression, and collagen I analyses. Body weight changes and mortality were observed. Light microscopy showed that lung fibrosis was minimal in TLR4(-/-) compared to that in WT mice on day 21 after bleomycin instillation. The Ashcroft score was significantly lower in TLR4(-/-) than in WT mice (3.667 ± 0.730 vs 4.945 ± 0.880, P < 0.05). Hydroxyproline content was significantly lower in TLR4(-/-) than in WT mice on day 21 after bleomycin injection (0.281 ± 0.022 vs 0.371 ± 0.047, P < 0.05). Compared to WT mice, bleomycin-treated TLR4(-/-) mice expressed significantly lower type I collagen mRNA levels (mesenchymal marker; 11.069 ± 2.627 vs 4.589 ± 1.440, P < 0.05). Collagen I was significantly lower in TLR4(-/-) than in WT mice (0.838 ± 0.352 vs 2.427 ± 0.551, P < 0.05). Bleomycin-treated TLR4(-/-) mice had a significantly lower mortality rate on day ...Continue Reading

Citations

Jul 10, 2016·Drug Delivery and Translational Research·Sonia Reimondez-TroitiñoMaría J Alonso
Nov 7, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Simone EbenerManuela Funke
Mar 4, 2020·FASEB BioAdvances·Jean PersonnazJean-Philippe Pradère
Oct 24, 2018·Molecular Imaging·Ying XiongGanghua Tang
Apr 5, 2018·Frontiers in Medicine·Omkar DesaiErica L Herzog
Apr 14, 2020·International Journal of Nanomedicine·Run DongHua-Ping Dai
Jan 21, 2021·International Journal of Molecular Sciences·Shiyi Tan, Shi Chen
Jun 24, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jay H LipinskiDavid N O'Dwyer
Aug 6, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Jiepeng WangNa Liu
Aug 27, 2021·Frontiers in Molecular Biosciences·Genta IshikawaErica L Herzog

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