Toll-like receptor 4 signaling in dysfunction of cardiac microvascular endothelial cells under hypoxia/reoxygenation.

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
Zheng ZhangFeng Cao

Abstract

This study was designed to detect the role of Toll-like receptor 4 (TLR4) signaling in the dysfunction of cardiac microvascular endothelial cells (CMECs) after hypoxia/reoxygenation (H/R). The cell viability of CMECs was measured by MTT assay. The migration of CMECs was detected by cell scratch wound assay. The expressions of TLR4, nuclear factor-kappa B (NF-κB) and eNOS were analyzed by Western blot. Secretions of nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were determined by NO detection kit and ELISA. Lipopolysaccharide (LPS) incubation increased the expressions of TLR4, NF-κB, IL-6 and TNF-α in CMECs (P < 0.05 vs. control). The CMECs after H/R injury had impaired cell viability (P < 0.01 vs. control) and migration ability (P < 0.05 vs. control). Moreover, the expressions of TLR4, NF-κB, IL-6 and TNF-α were elevated after H/R in CMECs (P < 0.01 vs. control), while NO and the eNOS expression were significantly decreased. In contrast, administration of the TLR4-neutralizing antibody MTS510 prior to H/R injury down-regulated the expressions of IL-6 and TNF-α and attenuated the dysfunction of CMECs. TLR4 and its signaling components can be activated by LPS and H/R in CMECs. Blocking the TLR...Continue Reading

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Citations

Jun 28, 2015·Microvascular Research·Juan ZhouChristian Lehmann
Mar 25, 2014·Journal of Ovarian Research·Luciana Lamarão DamousEdmund Chada Baracat
Feb 1, 2014·Free Radical Biology & Medicine·Jinyi WangHaichang Wang
Aug 22, 2017·Apoptosis : an International Journal on Programmed Cell Death·Zheng ZhangTaohong Hu
Nov 23, 2019·Cell and Tissue Research·Morteza HeidarzadehReza Rahbarghazi
Dec 9, 2020·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Masahito SatoJotaro Suzuki
Aug 15, 2021·Bioorganic & Medicinal Chemistry Letters·Shiyang ZhouJian Liu

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