Toll-like receptor 7 and 9 expression in peripheral blood mononuclear cells from patients with chronic hepatitis B and related hepatocellular carcinoma

Acta Pharmacologica Sinica
Ning XuZhi Chen

Abstract

The aim of the present study was to investigate the expression of Toll-like receptors (TLR) 7 and 9 in peripheral blood mononuclear cells (PBMC) of patients with chronic hepatitis B virus (HBV) infection and related hepatocellular carcinoma. The study group was comprised of 52 patients: 41 with chronic hepatitis B and 11 healthy controls. The protein and mRNA levels of TLR7 and TLR9 were evaluated using real-time PCR, Western blot analysis, and flow cytometry. We also detected the serum viral load of HBV in the patients and analyzed the correlation between HBV-DNA copies and the TLR expression. Our results demonstrated a lower TLR7 expression in all HBV infection groups compared to the controls. We found that HBV infection led to a decreased expression of TLR9 mRNA, but an increased expression of the TLR9 protein compared to the healthy group. The TLR protein levels are related to serum HBV-DNA (P<0.01). There are downregulations of TLR7 expression and TLR9 mRNA in PBMC of HBV-infected patients, but an increased TLR9 expression at the protein level.

References

Jan 1, 1995·Annual Review of Immunology·F V Chisari, C Ferrari
Mar 1, 1997·Electrophoresis·L Anderson, J Seilhamer
Feb 18, 1999·Molecular and Cellular Biology·S P GygiR Aebersold
May 11, 1999·Differentiation; Research in Biological Diversity·S McBrideM Clynes
Mar 17, 2000·Immunological Reviews·R Medzhitov, C Janeway
Feb 28, 2002·Annual Review of Immunology·Charles A Janeway, Ruslan Medzhitov
Apr 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Veit HornungGunther Hartmann
Jul 4, 2002·Molecular & Cellular Proteomics : MCP·Guoan ChenDavid G Beer
Jan 1, 2003·Veterinary Immunology and Immunopathology·Dirk Werling, Thomas W Jungi
Jan 14, 2003·Annual Review of Immunology·Kiyoshi TakedaShizuo Akira
Jul 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cynthia A LeiferDavid M Segal
Sep 30, 2004·Nature Immunology·Akiko Iwasaki, Ruslan Medzhitov
Dec 9, 2004·International Immunology·Kiyoshi Takeda, Shizuo Akira
Jan 6, 2005·Respiratory Research·Daniel DroemannTorsten Goldmann
May 14, 2005·Journal of Virology·Masanori IsogawaFrancis V Chisari
Sep 15, 2005·Biochemical and Biophysical Research Communications·Clive S McKimmieJohn K Fazakerley
Dec 6, 2005·International Journal of Pediatric Otorhinolaryngology·Margaret J LesmeisterMichael L Misfeldt
Dec 17, 2005·Nature Immunology·Stefan Bauer
Feb 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Erika SchlaepferRoberto F Speck
May 5, 2006·Seminars in Liver Disease·Kumar Visvanathan, Sharon R Lewin
Jul 20, 2006·Molecular Cancer Research : MCR·Melinda A MerrellKatri S Selander
Oct 24, 2006·Annals of the New York Academy of Sciences·Jongdae LeeEyal Raz
Nov 1, 2006·Laboratory Investigation; a Journal of Technical Methods and Pathology·Ying-Hsien HuangJiin-Haur Chuang

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Citations

Jun 10, 2010·Archivum Immunologiae Et Therapiae Experimentalis·Iwona Mozer-LisewskaJan Zeromski
Nov 17, 2010·Journal of Experimental & Clinical Cancer Research : CR·Zhong-Jun WuZhi-Qiang Wang
Oct 31, 2013·Archives of Pathology & Laboratory Medicine·Seyyed Mohammad Ali SajadiDerek Kennedy
Mar 1, 2011·Clinical Immunology : the Official Journal of the Clinical Immunology Society·David BreaJosé Castillo
Jun 19, 2013·Immunological Reviews·Christina C ChangSharon R Lewin
Jun 22, 2012·International Journal of Dermatology·Amira El Tawdy, Laila Rashed
Sep 6, 2013·The International Journal of Biochemistry & Cell Biology·Li-Jung KangSeong-Gene Lee
Apr 5, 2012·Gastroenterology Research and Practice·Megan CraneSharon R Lewin
Jun 7, 2014·Frontiers in Immunology·Nobuhiro Nakamoto, Takanori Kanai
Jul 5, 2016·Life Sciences·Zahra SepehriDerek Kennedy
Aug 19, 2017·Journal of Cancer Research and Clinical Oncology·Cecilia Martínez-CamposVicente Madrid-Marina
Feb 4, 2016·World Journal of Hepatology·Jorge André Gomes LopesPedro Pimentel-Nunes
Feb 6, 2017·Viral Immunology·Cecilia Martínez-CamposVicente Madrid-Marina
Jan 17, 2021·International Journal of Molecular Sciences·David DiazMelchor Alvarez-Mon

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