Toll-like Receptor 7 Is Reduced in Severe Asthma and Linked to an Altered MicroRNA Profile

American Journal of Respiratory and Critical Care Medicine
Hitasha RupaniTilman Sanchez-Elsner

Abstract

Asthma is one of the most common chronic diseases worldwide, and individuals with severe asthma experience recurrent exacerbations. Exacerbations are predominantly viral associated and have been linked to defective airway IFN responses. Ascertaining the molecular mechanisms underlying this deficiency is a major research goal to identify new therapeutic targets. We investigated the hypothesis that reduced Toll-like receptor 7 (TLR7)-derived signaling drove the impaired IFN responses to rhinovirus by asthmatic alveolar macrophages (AMs); the molecular mechanisms underlying this deficiency were explored. AMs were recovered from bronchoalveolar lavage from healthy subjects and patients with severe asthma. Expression of pattern-recognition receptors and microRNAs was evaluated by quantitative polymerase chain reaction and Western blotting. A TLR7-luciferase reporter construct was created to evaluate binding of microRNAs to the 3' untranslated region of TLR7. IFN production was measured by quantitative polymerase chain reaction and ELISA. The expression of TLR7 was significantly reduced in severe asthma AMs and was associated with reduced rhinovirus and imiquimod-induced IFN responses by these cells compared with healthy AMs. Severe ...Continue Reading

References

Oct 16, 1993·BMJ : British Medical Journal·K G NicholsonD C Ireland
Nov 26, 1999·The European Respiratory Journal·E F JuniperD R King
Feb 7, 2001·Virology·M G RossmannT S Baker
Oct 5, 2001·Clinical Microbiology Reviews·C E Samuel
Feb 15, 2002·The European Respiratory Journal·P GodardG Duru
Feb 21, 2002·The European Respiratory Journal·P A B WarkP G Gibson
Mar 29, 2002·The Journal of Infectious Diseases·Anne G MosserJames E Gern
May 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jongdae LeeHoward B Cottam
Feb 21, 2004·Science·Sandra S DieboldCaetano Reis e Sousa
Mar 23, 2005·The Journal of Experimental Medicine·Peter A B WarkDonna E Davies
May 17, 2005·American Journal of Respiratory and Critical Care Medicine·Terry V GrissellPeter G Gibson
Jul 26, 2005·American Journal of Respiratory and Critical Care Medicine·Mai-Lan N HuynhSally E Wenzel
Oct 8, 2005·American Journal of Respiratory Cell and Molecular Biology·Yin ChenReen Wu
Apr 1, 2006·American Journal of Respiratory and Critical Care Medicine·Charles S BerensonSanjay Sethi
Aug 15, 2006·Nature Medicine·Marco ContoliSebastian L Johnston
Aug 2, 2007·Immunology and Cell Biology·Alex J V Thompson, Stephen A Locarnini
Sep 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Simon D MessageSebastian L Johnston
Mar 7, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nicole BedkeDonna E Davies
Jun 9, 2009·American Journal of Respiratory Cell and Molecular Biology·Albert P SenftKevin S Harrod
Aug 1, 2009·The European Respiratory Journal·M RoponenJ W Upham
Jan 27, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alexander PandaAlbert C Shaw
Apr 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·John DeVincenzoAkshay Vaishnaw
Jun 16, 2010·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·N L Korpi-SteinerP J Bertics
Sep 18, 2010·The Journal of Biological Chemistry·Lindsay K SmithJohn A Cidlowski
Oct 5, 2010·American Journal of Respiratory and Critical Care Medicine·Patrick MalliaSebastian L Johnston
Jun 10, 2011·International Archives of Allergy and Immunology·Katsuhito IikuraKenji Matsumoto
Feb 14, 2012·Current Opinion in Virology·John W Schoggins, Charles M Rice
Jun 9, 2012·American Journal of Respiratory Cell and Molecular Biology·Melanie J JardimDavid Diaz-Sanchez
Jul 14, 2012·The European Respiratory Journal·Hitasha RupaniPeter Howarth
Sep 8, 2012·American Journal of Respiratory and Critical Care Medicine·Owen D SolbergPrescott G Woodruff
Dec 4, 2012·The Journal of Infection·Giel R GaajetaanFrank R Stassen
Mar 29, 2013·The New England Journal of Medicine·Harry L A JanssenMichael R Hodges
Oct 16, 2013·Thorax·Annemarie SykesSebastian L Johnston
Oct 18, 2013·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K S ParsonsP A B Wark
Jan 15, 2014·Allergy·A CustovicN G Papadopoulos

❮ Previous
Next ❯

Citations

Jun 9, 2016·Mediators of Inflammation·Zhiyong DongFang Wang
Mar 8, 2017·Pharmacological Reports : PR·Yoshihiko ChibaHiroyasu Sakai
Nov 29, 2016·The Lancet. Respiratory Medicine·Shweta Sood, Mario Castro
Feb 25, 2017·Current Allergy and Asthma Reports·Regina K Rowe, Michelle A Gill
Dec 23, 2016·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C AkotoE J Swindle
May 4, 2017·The European Respiratory Journal·Michael R EdwardsUNKNOWN members of the EARIP WP2 working group
Dec 28, 2018·Journal of the Pediatric Infectious Diseases Society·Regina K Rowe, Michelle A Gill
Oct 3, 2019·American Journal of Respiratory and Critical Care Medicine·Sebastian L Johnston
Jun 3, 2017·Clinical Epigenetics·David A ArmstrongAlix Ashare
Nov 20, 2019·Allergy, Asthma & Immunology Research·Styliani TakaNikolaos G Papadopoulos
May 28, 2019·Immunological Investigations·Krzysztof SpecjalskiEwa Jassem
Sep 13, 2020·Nature Reviews. Immunology·Harald Renz, Chrysanthi Skevaki
Jul 2, 2016·American Journal of Respiratory and Critical Care Medicine·Sebastian L Johnston
May 23, 2017·American Journal of Respiratory and Critical Care Medicine·Bianca BeghéAlberto Papi
Oct 23, 2018·F1000Research·Spyridon Makris, Sebastian Johnston
Jun 27, 2019·Journal of Cellular Biochemistry·Yang WangYaolin Wang
Apr 8, 2020·American Journal of Respiratory and Critical Care Medicine·Jose L GomezGeoffrey L Chupp
Nov 26, 2020·Immunity, Inflammation and Disease·Ting-Yu LinHorng-Chyuan Lin
May 1, 2017·Pharmacological Reports : PR·Yoshihiko ChibaHiroyasu Sakai
Jan 26, 2021·The Journal of Allergy and Clinical Immunology·Alisha M SmithSunil K Ahuja
Jun 24, 2021·Clinical and Translational Allergy·Mohammed A KyyalyRamesh J Kurukulaaratchy
Dec 18, 2018·Journal of Innate Immunity·Stanislas Grassin-DelylePhilippe Devillier

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

American Thoracic Association Journals

Discover the latest respiratory research published by the journals from the American Thoracic Society.

Acute viral rhinopharyngitis

Acute viral rhinopharyngitis, also known as "common cold", is an acute, self-limiting viral infection of the upper respiratory tract involving the nose, sinuses, pharynx and larynx. Discover the latest research on acute viral rhinopharyngitis here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.