Toll-like receptor 9 and vascular endothelial growth factor levels in human kidneys from lupus nephritis patients

Journal of Nephrology
Marianne FrieriSofia Rubinstein

Abstract

In the lupus mouse and systemic lupus erythematosus (SLE) patients, DNA fragments isolated from plasma may mimic microbial DNA and trigger Toll-like receptor 9 (TLR9) signaling with formation of autoantibodies against DNA fragments and nucleosomes. Vascular endothelial growth factor (VEGF) is a tightly regulated angiogenic cytokine in the kidney. The present study investigated glomerular and tubular expression of both TLR9 and VEGF in biopsies from human subjects with lupus nephritis (LN) and normal controls. Kidney biopsies in LN (n=8) and normal controls (n=10) were evaluated for expression of TLR9 and VEGF. The degree of kidney damage was analyzed according to the International Society of Nephrology / Renal Pathology Society classification. Immunohistochemistry was performed, and slides were incubated with antibodies against VEGF and TLR9 monoclonal antibody, stained with hematoxylin and eosin, mounted and microscopically scored at ×10 and ×20. We observed intense staining of glomeruli and tubules for TLR9 up to 3+ from patients with LN. Samples from LN subjects showed 3+ staining of glomeruli but only up to 2+ in tubules for VEGF. There was less significant staining for TLR9 and none for VEGF in controls. There was no corre...Continue Reading

Citations

Apr 1, 2015·Nature Reviews. Nephrology·Chandra Mohan, Chaim Putterman
Sep 8, 2012·Current Allergy and Asthma Reports·Adeel Zubair, Marianne Frieri
Sep 4, 2012·Current Allergy and Asthma Reports·Saad SyedMarianne Frieri
Nov 16, 2013·Current Allergy and Asthma Reports·Adeel Zubair, Marianne Frieri
Nov 11, 2016·BMC Nephrology·Sirirat AnutrakulchaiRichard J Johnson
Apr 14, 2018·Journal of Biomedical Science·Satish Kumar Devarapu, Hans-Joachim Anders
Mar 15, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Robyn E MillsMichelle L Hermiston
Nov 27, 2021·Journal of Nephrology·Vincenzo SepeTeresa Rampino

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