Toll-like Receptor Expression Profile of Human Dental Pulp Stem/Progenitor Cells

Journal of Endodontics
Karim M Fawzy El-SayedChristof E Dörfer

Abstract

Human dental pulp stem/progenitor cells (DPSCs) show remarkable regenerative potential in vivo. During regeneration, DPSCs may interact with their inflammatory environment via toll-like receptors (TLRs). The present study aimed to depict for the first time the TLR expression profile of DPSCs. Cells were isolated from human dental pulp, STRO-1-immunomagnetically sorted, and seeded out to obtain single colony-forming units. DPSCs were characterized for CD14, CD34, CD45, CD73, CD90, CD105, and CD146 expression and for their multilineage differentiation potential. After incubation of DPSCs in basic or inflammatory medium (interleukin-1β, interferon-γ, interferon-α, tumor necrosis factor-α), TLR expression profiles were generated (DPSCs and DPSCs-i). DPSCs showed all characteristics of stem/progenitor cells. In basic medium DPSCs expressed TLRs 1-10 in different quantities. The inflammatory medium upregulated the expression of TLRs 2, 3, 4, 5, and 8, downregulated TLRs 1, 7, 9, and 10, and abolished TLR6. The current study describes for the first time the distinctive TLR expression profile of DPSCs in uninflamed and inflamed conditions.

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Citations

May 2, 2019·Journal of Cellular Physiology·Keisuke NaraTakashi Okiji
Jun 11, 2019·Tissue Engineering. Part B, Reviews·Karim M Fawzy El-SayedChristof E Dörfer
May 31, 2020·Journal of Clinical Periodontology·Mohamed MekhemarKarim Fawzy El-Sayed
Apr 6, 2017·BioMed Research International·Aileen I TsaiTzung-Hai Yen
Mar 16, 2019·Scientific Reports·Matthias WidbillerKerstin M Galler
Mar 19, 2020·Stem Cells International·Geraldine M AhmedKarim Fawzy El-Sayed
Aug 9, 2020·Frontiers in Cell and Developmental Biology·Noemi EiroFrancisco J Vizoso
Mar 19, 2020·Stem Cells International·Li-Li ZhouK M Fawzy El-Sayed
Dec 21, 2019·Advanced Pharmaceutical Bulletin·Faramarz RahmatizadehMaryam Pashaiasl
Apr 2, 2020·Molecular Genetics & Genomic Medicine·Ba Hoang Anh MaiGérard Aboudharam
Dec 16, 2020·Polymers·Marwa M S AbbassKarim M Fawzy El-Sayed
Feb 6, 2021·International Journal of Molecular Sciences·Kerstin M GallerMarkus Feuerer

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