Toll-like receptor genes are differentially expressed at the sites of infection during the progression of Johne's disease in outbred sheep

Veterinary Immunology and Immunopathology
Deborah L TaylorR J Whittington

Abstract

Toll-like receptors (TLR) are engaged by ligands on microbial pathogens to initiate innate and adaptive immune responses. Little is known about TLR involvement during infection with Mycobacterium avium subsp. paratuberculosis (M. ptb), the cause of Johne's disease in ruminants, although there is a profound immunopathological response in affected animals. We have analyzed the expression of 10 TLR genes relative to validated reference genes at predilection sites in ileum, jejunum and associated lymph nodes as well as in peripheral blood, to determine if TLR expression is altered in response to infection with M. ptb in outbred sheep. Previously unexposed animals from two flocks and animals from three naturally infected flocks were used with restricted maximum likelihood linear mixed modeling applied to determine significant differences. These were related to the pathologies observed at different stages of infection in exposed sheep, after allowing for other sources of variation. In most cases there were differences in TLR expression between early paucibacillary and multibacillary groups when compared to uninfected sheep, with most TLRs for the paucibacillary group having lower expression levels than the multibacillary group. Incre...Continue Reading

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Citations

Apr 2, 2013·Research in Veterinary Science·Shyamala ThirunavukkarasuRichard J Whittington
Nov 15, 2011·Veterinary Immunology and Immunopathology·Supatsak SubharatD Neil Wedlock
Dec 17, 2011·International Journal of Molecular Sciences·Simone PelettoPier Luigi Acutis
Jun 1, 2013·International Journal of Molecular Sciences·William R VorachekJean A Hall
Feb 1, 2018·Archives of Microbiology·Lmar Babrak, Luiz E Bermudez
Dec 12, 2012·Clinical and Vaccine Immunology : CVI·Chandrashekhar CharavaryamathPhilip J Griebel
Jan 11, 2011·Comparative Immunology, Microbiology and Infectious Diseases·Auriol C PurdieRichard J Whittington
Jul 3, 2021·Animals : an Open Access Journal From MDPI·Palazzo FiorentinaGiulietta Minozzi

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