Toll-like receptors 2 and 4 modulate intestinal IL-10 differently in ileum and colon

United European Gastroenterology Journal
Eva LatorreJosé E Mesonero

Abstract

Inflammatory bowel diseases are consequence of an intestinal homeostasis breakdown in which innate immune dysregulation is implicated. Toll-like receptor (TLR)2 and TLR4 are immune recognition receptors expressed in the intestinal epithelium, the first physical-physiological barrier for microorganisms, to inform the host of the presence of Gram-positive and Gram-negative organisms. Interleukin (IL)-10 is an essential anti-inflammatory cytokine that contributes to maintenance of intestinal homeostasis. Our main aim was to investigate intestinal IL-10 synthesis and release, and whether TLR2 and TLR4 are determinants of IL-10 expression in the intestinal tract. We used Caco-2 cell line as an enterocyte-like cell model, and also ileum and colon from mice deficient in TLR2, TLR4 or TLR2/4 to test the involvement of TLR signaling. Intestinal epithelial cells are able to synthesize and release IL-10 and their expression is increased after TLR2 or TLR4 activation. IL-10 regulation seems to be tissue specific, with IL-10 expression in the ileum regulated by a compensation between TLR2 and TLR4 expression, whereas in the colon, TLR2 and TLR4 affect IL-10 expression independently. Intestinal epithelial cells could release IL-10 in respons...Continue Reading

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Citations

Oct 17, 2017·Journal of Cellular Physiology·Elena LayuntaJosé E Mesonero
Apr 27, 2019·Frontiers in Immunology·Renata CurciarelloCecilia Isabel Muglia
May 28, 2019·Frontiers in Immunology·Alessandro PoggiMaria Raffaella Zocchi
Dec 11, 2019·International Journal of Molecular Sciences·Xiang Xue, Daniel M Falcon
Jan 27, 2019·Nutrients·Annelies VerlaetMalgorzata Teodorowicz
Jun 17, 2020·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Berta BueyLourdes Sánchez
Jul 6, 2021·Frontiers in Immunology·Stamatia PapoutsopoulouBarry J Campbell
Jul 17, 2021·Cellular and Molecular Gastroenterology and Hepatology·Huong D NguyenAndrew W Stadnyk

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

Prism GraphPad
GraphPad
Quantity One Analysis

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