Toll-like receptors involved in the pathogenesis of experimental Candida albicans keratitis.

Investigative Ophthalmology & Visual Science
Xiaoyong Yuan, Kirk R Wilhelmus

Abstract

Purpose. To investigate the expression and function of toll-like receptors (TLRs) during experimental keratomycosis. Methods. Scarified corneas of BALB/c mice were topically inoculated with Candida albicans and compared with control corneas by a murine gene microarray and immunostaining. Real-time reverse transcription polymerase chain reaction (RT-PCR) determined relative TLR gene expression in murine and human donor corneas. The scarified corneas of TLR2(-/-) mice, TLR4(-/-) mice, and C57BL/6J control mice were also inoculated with C. albicans, to determine relative severity, fungal load, and cytokine transcript levels. Results. TLR1, -2, -4, -6, and -13 were significantly upregulated (5- to 10-fold; P < 0.01) by microarray, and TLR1, -2, -4, and -13 were significantly increased (4- to 11-fold; P < 0.05) by real-time RT-PCR in BALB/c murine corneas. Similarly, TLR2, -6, and -13 were significantly upregulated (5- to 16-fold; P < or = 0.001) by real-time RT-PCR in C57BL/6J murine corneas the day after inoculation, and TLR2 and -13 remained significantly (P < 0.05) increased after 1 week. TLR2 transcript was also upregulated twofold (P = 0.04) in C. albicans-inoculated explanted human corneas. Although murine keratitis severity ...Continue Reading

Citations

Aug 11, 2011·The Journal of Infectious Diseases·Rajapandian Sivaganesa KarthikeyanPrajna Lalitha
Dec 14, 2011·PloS One·Billy D NovosadMichelle C Callegan
Dec 17, 2009·Experimental Eye Research·Xiaoyong YuanKirk R Wilhelmus
May 23, 2012·Cell Host & Microbe·Nydiaris Hernández-Santos, Sarah L Gaffen
Jul 22, 2015·Infection and Immunity·Salai Madhumathi ParkunanMichelle C Callegan
Feb 7, 2018·Scientific Reports·Hyun Jung ParkChan Hum Park
Dec 17, 2010·Current Eye Research·Ayse Kalkanci, Sengul Ozdek
Jul 10, 2019·Scientific Reports·Xiaohua LiShikun He
Jun 5, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiaoyu JiangLinda D Hazlett
Jan 1, 2015·3 Biotech·Amandeep KaurVirender Kumar
Jun 11, 2021·The Journal of Veterinary Medical Science·Takahiro AyaTakashi Takeuchi
Jun 15, 2021·The Journal of Veterinary Medical Science·Takahiro AyaTakashi Takeuchi
Jun 10, 2011·Current Opinion in Ophthalmology

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