Abstract
Trichloroethylene (TCE) is extensively used in commercial applications, despite its risk to human health via soil and groundwater contamination. The stability of TCE, which is a useful characteristic for commercial application, makes it difficult to remove it from the environment. Numerous studies have demonstrated that TCE can be effectively removed from the environment using bioremediation. Pseudomonas putida F1 is capable of degrading TCE into less hazardous byproducts via the toluene dioxygenase pathway (TOD). Unfortunately, these bioremediation systems are not self-sustaining, as the degradation capacity declines over time. Fortunately, the replacement of metabolic co-factors is sufficient in many cases to maintain effective TCE degradation. Thus, monitoring systems must be developed to predict when TCE degradation rates are likely to decline. Herein, we show evidence that tod expression levels correlate with the ability of P. putida F1 to metabolize TCE in the presence of toluene. Furthermore, the presence of toluene improves the replication of P. putida F1, even when TCE is present at high concentration. These findings may be applied to real world applications to decide when the bioremediation system requires supplementa...Continue Reading
References
Jan 31, 1975·Annals of the New York Academy of Sciences·C Maltoni, G Lefemine
Jan 1, 1991·Annual Review of Microbiology·B D Ensley
Nov 22, 2001·Journal of Bacteriology·S J VandecasteeleJ Van Eldere
Jul 26, 2002·Analytical Biochemistry·Ronald H Lekanne DeprezAntoon F M Moorman
May 2, 2003·Journal of Medical Microbiology·Hakan SavliHaluk Vahaboglu
May 7, 2004·Applied and Environmental Microbiology·Yuki MoronoKatsutoshi Hori
Jun 9, 2004·Applied and Environmental Microbiology·Axel FeyCarlos A Guzmán
Aug 6, 2004·Applied and Environmental Microbiology·Matthias LabrenzManfred G Höfle
Oct 20, 2005·Journal of Bioscience and Bioengineering·Shaila KabirKiminori Itoh
Feb 10, 2006·Biodegradation·Bilge Alpaslan Kocamemi, Ferhan Ceçen
Feb 14, 2006·Molecular Aspects of Medicine·Simone Fleige, Michael W Pfaffl
Dec 1, 2006·Applied Microbiology and Biotechnology·Sergio BordelSantiago Villaverde
Jun 6, 2007·Water Science and Technology : a Journal of the International Association on Water Pollution Research·B Alpaslan Kocamemi, F Ceçen
Jul 1, 2008·Chemosphere·Yan-Min ChenJui-Che Lin
Jun 1, 1995·Environmental Science & Technology·G D Hopkins, P L McCarty
Citations
May 27, 2014·Journal of Hazardous Materials·Liang ChenSong Jin
Feb 14, 2016·Journal of Hazardous Materials·João JesusAnthony S Danko
Sep 16, 2014·Environmental Technology·Hui LiYong-di Liu
Jan 23, 2017·The Science of the Total Environment·Qihong LuHojae Shim
Nov 4, 2017·Environmental Technology·Agnieszka TabernackaMarcin Żołądek