Toluene Elicits a Carbon Starvation Response in Pseudomonas putida mt-2 Containing the TOL Plasmid pWW0.

Applied and Environmental Microbiology
P Vercellone-Smith, D S Herson

Abstract

Pseudomonas putida mt-2(pWWO) exhibited a carbon starvation response in the presence of toluene, a utilizable carbon source. When growth-supporting (4-mg/liter), inhibitory (130-mg/liter), and lethal (267-mg/ liter) levels of toluene were provided as the sole carbon source, P. putida responded by rapidly inhibiting protein synthesis and by producing 26 new proteins, 22 of which overlapped with those induced by carbon starvation. P. putida produced the same proteins when cultures were starved by depleting their carbon source or were downshifted into a carbon-free medium. Carbon supplementation of toluene-exposed cells suppressed the production of the toluene-induced proteins. The level of toluene provided as the sole carbon source influenced the length of time that this response was observed. Following 1.5 to 3 h in a basal salts medium with 4 mg of toluene per liter, protein synthesis increased, the production of the majority of the toluene-induced proteins ceased, and the cells began to grow. In cells provided with 130 mg of toluene per liter, protein synthesis remained inhibited over a 6.5-h experimental period. At this concentration, the production of 15 toluene-induced proteins was prolonged, with nine still detectable in t...Continue Reading

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Citations

Jan 25, 2011·Microbial Biotechnology·Prashanth BhagannaJohn E Hallsworth
May 10, 2002·Journal of Contaminant Hydrology·Eric A SeagrenAlbert J Valocchi
Jan 18, 2006·Biotechnology Advances·Francisco P ChávezCarlos A Jerez
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Jun 15, 2007·Journal of Microbiological Methods·Susanne GüntherSusann Müller

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