Tomato Male sterile 1035 is essential for pollen development and meiosis in anthers

Journal of Experimental Botany
Hee-Jin JeongByoung-Cheorl Kang

Abstract

Male fertility in flowering plants depends on proper cellular differentiation in anthers. Meiosis and tapetum development are particularly important processes in pollen production. In this study, we showed that the tomato male sterile (ms10(35)) mutant of cultivated tomato (Solanum lycopersicum) exhibited dysfunctional meiosis and an abnormal tapetum during anther development, resulting in no pollen production. We demonstrated that Ms10(35) encodes a basic helix-loop-helix transcription factor that is specifically expressed in meiocyte and tapetal tissue from pre-meiotic to tetrad stages. Transgenic expression of the Ms10(35) gene from its native promoter complemented the male sterility of the ms10(35) mutant. In addition, RNA-sequencing-based transcriptome analysis revealed that Ms10(35) regulates 246 genes involved in anther development processes such as meiosis, tapetum development, cell-wall degradation, pollen wall formation, transport, and lipid metabolism. Our results indicate that Ms10(35) plays key roles in regulating both meiosis and programmed cell death of the tapetum during microsporogenesis.

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Citations

Oct 8, 2015·Trends in Plant Science·Jianxin ShiDabing Zhang
Jul 14, 2018·The Plant Journal : for Cell and Molecular Biology·Fernando Pérez-MartínRafael Lozano
Feb 9, 2019·Plant Molecular Biology·Zengyu GanZhenhai Han
Nov 21, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Shuai SunXia Cui
Jan 11, 2020·The Plant Journal : for Cell and Molecular Biology·Minmin DuChuanyou Li
Dec 22, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Qing ChengHuolin Shen
Jan 14, 2017·Plant & Cell Physiology·Shuhei HaoHiroshi Ezura
Dec 14, 2018·International Journal of Molecular Sciences·Bingli DingQiong Hu
Apr 26, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Xue CaoZejun Huang
Jun 2, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Qing ChengHuolin Shen
Feb 6, 2020·Frontiers in Plant Science·Xiaoning Lei, Bing Liu
Nov 1, 2015·The Plant Genome·Vahid OmidvarMartin Fellner
Jul 16, 2021·Frontiers in Plant Science·Abate Mekonnen SolomonByoung-Cheorl Kang

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Methods Mentioned

BETA
transmission electron microscopy
scanning electron microscopy
PCR
RNA-seq
light microscopy
electrophoretic mobility shift assay

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