Tomato phospholipid hydroperoxide glutathione peroxidase inhibits cell death induced by Bax and oxidative stresses in yeast and plants

Plant Physiology
Shaorong ChenMartin B Dickman

Abstract

Using a conditional life or death screen in yeast, we have isolated a tomato (Lycopersicon esculentum) gene encoding a phospholipid hydroperoxide glutathione peroxidase (LePHGPx). The protein displayed reduced glutathione-dependent phospholipid hydroperoxide peroxidase activity, but differs from counterpart mammalian enzymes that instead contain an active seleno-Cys. LePHGPx functioned as a cytoprotector in yeast (Saccharomyces cerevisiae), preventing Bax, hydrogen peroxide, and heat stress induced cell death, while also delaying yeast senescence. When tobacco (Nicotiana tabacum) leaves were exposed to lethal levels of salt and heat stress, features associated with mammalian apoptosis were observed. Importantly, transient expression of LePHGPx protected tobacco leaves from salt and heat stress and suppressed the apoptotic-like features. As has been reported, conditional expression of Bax was lethal in tobacco, resulting in tissue collapse and membrane permeability to Evans blue. When LePHGPx was coexpressed with Bax, little cell death and no vital staining were observed. Moreover, stable expression of LePHGPx in tobacco conferred protection against the fungal phytopathogen Botrytis cinerea. Taken together, our data indicated th...Continue Reading

References

Jan 1, 1990·Methods in Enzymology·M MaiorinoF Ursini
Feb 1, 1989·Journal of Virological Methods·J L White, J M Kaper
Mar 29, 1985·Biochimica Et Biophysica Acta·F UrsiniC Gregolin
Jun 1, 1983·European Journal of Biochemistry·O EppA Wendel
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·W WangM B Dickman
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·D L Vaux, A Strasser
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·V N GladyshevT C Stadtman
Jan 1, 1996·Annual Review of Biochemistry·T C Stadtman
Jun 13, 1998·The Journal of Biological Chemistry·J Cai, D P Jones
Jun 17, 1998·The Plant Journal : for Cell and Molecular Biology·T W McNellisB J Staskawicz
Feb 3, 1999·The Plant Cell·D A Navarre, T J Wolpert
Mar 13, 1999·The Journal of Biological Chemistry·A C LeeT Finkel
May 20, 1999·The Journal of Cell Biology·F MadeoK U Fröhlich
May 25, 1999·Protein Science : a Publication of the Protein Society·O EmanuelssonG von Heijne
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·C Lacomme, S Santa Cruz
Jul 15, 1999·Biochemical and Biophysical Research Communications·W TaoJ I Morgan
May 10, 2000·FEBS Letters·K U Fröhlich, F Madeo
Jun 22, 2000·The Journal of Biological Chemistry·S C KampranisA M Makris
Nov 9, 2000·The Plant Journal : for Cell and Molecular Biology·F L TakkenM H Joosten
Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·M B DickmanR French
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·M Kawai-YamadaH Uchimiya
Oct 20, 2001·Current Opinion in Plant Biology·E P Beers, J M McDowell
Nov 8, 2001·Current Opinion in Cell Biology·M Serrano, M A Blasco
Jan 10, 2002·Biochemical and Biophysical Research Communications·Haejeong MoonDae-Jin Yun

❮ Previous
Next ❯

Citations

Dec 14, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Chang-Jiang ZhaoGuo-Dong Lu
Apr 1, 2008·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Amanjot Singh, Anil Grover
Mar 22, 2005·Biochimica Et Biophysica Acta·Xiao-Dong YangJin-Yuan Liu
Apr 27, 2005·FEMS Yeast Research·Rieka ReekmansRoland Contreras
Nov 17, 2009·Cell Death and Differentiation·M T Greenwood, P Ludovico
May 21, 2011·Cell Death and Differentiation·T IshikawaE Lam
Mar 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Brett WilliamsMartin B Dickman
Jan 13, 2010·PloS One·Xinwei ChenRobbie Waugh
Jun 23, 2012·Journal of Plant Physiology·Emma Fernández-CrespoPilar García-Agustín
Apr 25, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Prakash M Gopalakrishnan NairJinhee Choi
Mar 17, 2010·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Mian WangBaohua Xu
Jan 14, 2009·Seminars in Cancer Biology·Nathan SusnowDavid M Hockenbery
Aug 19, 2008·Molecular Plant Pathology·Brett Williams, Marty Dickman
Jul 1, 2006·Molecular Plant Pathology·Jafargholi ImaniRalph Hückelhoven
Apr 11, 2007·The FEBS Journal·Stéphane HerbetteJoël R Drevet
Jan 20, 2010·Plant, Cell & Environment·Jean-Luc Cacas
Mar 6, 2013·Molecular Plant Pathology·Chang-Ming YeJeanmarie Verchot
Dec 15, 2010·Biochimica Et Biophysica Acta·Drew WilliamsMichael T Greenwood
Apr 22, 2015·Journal of Proteomics·Marta Serra-SorianoVicente Pallás
Mar 19, 2013·Plant Science : an International Journal of Experimental Plant Biology·Anil GroverDhruv Lavania
Feb 5, 2008·Biochimica Et Biophysica Acta·Chamel M Khoury, Michael T Greenwood
Oct 26, 2010·Biochimica Et Biophysica Acta·Liam PorttMichael T Greenwood
Mar 22, 2008·FEMS Yeast Research·Chamel M KhouryMichael T Greenwood
Nov 17, 2017·Molecular Plant-microbe Interactions : MPMI·Mingsheng QiSteven A Whitham

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis