Tomato root microbiota and Phytophthora parasitica-associated disease

Microbiome
Marie LarousseEric Galiana

Abstract

Interactions between pathogenic oomycetes and microbiota residing on the surface of the host plant root are unknown, despite being critical to inoculum constitution. The nature of these interactions was explored for the polyphagous and telluric species Phytophthora parasitica. Composition of the rhizospheric microbiota of Solanum lycopersicum was characterized using deep re-sequencing of 16S rRNA gene to analyze tomato roots either free of or partly covered with P. parasitica biofilm. Colonization of the host root surface by the oomycete was associated with a shift in microbial community involving a Bacteroidetes/Proteobacteria transition and Flavobacteriaceae as the most abundant family. Identification of members of the P. parasitica-associated microbiota interfering with biology and oomycete infection was carried out by screening for bacteria able to (i) grow on a P. parasitica extract-based medium (ii), exhibit in vitro probiotic or antibiotic activity towards the oomycete (iii), have an impact on the oomycete infection cycle in a tripartite interaction S. lycopersicum-P. parasitica-bacteria. One Pseudomonas phylotype was found to exacerbate disease symptoms in tomato plants. The lack of significant gene expression response ...Continue Reading

References

Oct 6, 1999·Genome Research·X Huang, A Madan
Sep 30, 2000·Applied and Environmental Microbiology·K PicardN Benhamou
Apr 12, 2003·Journal of Clinical Microbiology·G DevulderJ P Flandrois
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Jun 18, 2004·Immunological Reviews·Thorsten NürnbergerLizelle Piater
Nov 17, 2006·Nature·Jonathan D G Jones, Jeffery L Dangl
May 18, 2007·Annual Review of Microbiology·Thomas Danhorn, Clay Fuqua
Jun 26, 2007·Applied and Environmental Microbiology·Qiong WangJames R Cole
Jul 3, 2007·Ecology·Noah FiererRobert B Jackson
Aug 17, 2010·Bioinformatics·Robert C Edgar
Dec 9, 2010·BMC Microbiology·Ping KongChuanxue Hong
May 10, 2011·Science·Rodrigo MendesJos M Raaijmakers
May 11, 2011·The Plant Journal : for Cell and Molecular Biology·Johana C Misas-VillamilRenier A L van der Hoorn
May 20, 2011·Nucleic Acids Research·Robert D FinnSean R Eddy
Jun 28, 2011·Bioinformatics·Robert C EdgarRob Knight
Jul 12, 2011·Applied and Environmental Microbiology·Eric GalianaMichel Ponchet
Dec 1, 2011·Microbiology and Molecular Biology Reviews : MMBR·P Frey-KlettA Sarniguet
Feb 5, 2013·Annual Review of Plant Biology·Davide BulgarelliPaul Schulze-Lefert
Apr 5, 2013·PLoS Pathogens·Johana C Misas-VillamilRenier A L van der Hoorn
May 23, 2013·Environmental Science and Pollution Research International·J GerboreP Rey
Sep 24, 2013·Nature Reviews. Microbiology·Laurent PhilippotWim H van der Putten
Nov 30, 2013·Nucleic Acids Research·James R ColeJames M Tiedje
Mar 19, 2014·Frontiers in Cellular and Infection Microbiology·Muriel Vayssier-TaussatThierry Candresse
Mar 29, 2014·The ISME Journal·Yiying LiuJos M Raaijmakers
May 7, 2014·Cellular Microbiology·Charisse Petersen, June L Round
May 23, 2014·Current Opinion in Plant Biology·Eric Kemen
Jul 8, 2014·Frontiers in Plant Science·Sarah L Lebeis
Aug 5, 2014·Fungal Biology·Pieter van West, Gordon W Beakes
Sep 2, 2014·TheScientificWorldJournal·Eiko E KuramaeJohannes A van Veen
Mar 4, 2015·Cell Host & Microbe·Davide BulgarelliPaul Schulze-Lefert
Oct 10, 2015·Current Opinion in Genetics & Development·Suomeng DongSophien Kamoun
Nov 9, 2015·Journal of Experimental Botany·Gabriele BergMartina Köberl
Apr 22, 2016·Microbes and Environments·Tomoki NishiokaMasafumi Shimizu
Jan 27, 2017·PLoS Pathogens·Marie Larousse, Eric Galiana

❮ Previous
Next ❯

Citations

Mar 29, 2018·Microbiome·M Amine HassaniStéphane Hacquard
Feb 26, 2020·Microbiome·Jean-Claude OgierSophie Gaudriault
Dec 12, 2020·Computational and Structural Biotechnology Journal·Ilaria BassaniEric Galiana
Jun 4, 2021·Microbial Biotechnology·Guangfei WeiShilin Chen

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCR
fluorescence microscopy
PCA
dissection

Software Mentioned

UCHIIME
FastQC
Phyloseq
PRINSEQ
AxioVision
CAP3
ZEN
InViewTM
PhyML
Seaview4

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.