Tomato yellow leaf curl virus infection of a resistant tomato line with a silenced sucrose transporter gene LeHT1 results in inhibition of growth, enhanced virus spread, and necrosis.

Planta
Assaf EybishtzHenryk Czosnek

Abstract

To identify genes involved in resistance of tomato to Tomato yellow leaf curl virus (TYLCV), cDNA libraries from lines resistant (R) and susceptible (S) to the virus were compared. The hexose transporter LeHT1 was found to be expressed preferentially in R tomato plants. The role of LeHT1 in the establishment of TYLCV resistance was studied in R plants where LeHT1 has been silenced using Tobacco rattle virus-induced gene silencing (TRV VIGS). Following TYLCV inoculation, LeHT1-silenced R plants showed inhibition of growth and enhanced virus accumulation and spread. In addition, a necrotic response was observed along the stem and petioles of infected LeHT1-silenced R plants, but not on infected not-silenced R plants. This response was specific of R plants since it was absent in infected LeHT1-silenced S plants. Necrosis had several characteristics of programmed cell death (PCD): DNA from necrotic tissues presented a PCD-characteristic ladder pattern, the amount of a JNK analogue increased, and production of reactive oxygen was identified by DAB staining. A similar necrotic reaction along stem and petioles was observed in LeHT1-silenced R plants infected with the DNA virus Bean dwarf mosaic virus and the RNA viruses Cucumber mosai...Continue Reading

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Jun 7, 2012·Protoplasma·Pranav Pankaj SahuManoj Prasad
Mar 24, 2015·Briefings in Functional Genomics·Hernan G Rosli, Gregory B Martin
Sep 20, 2017·Plant & Cell Physiology·Benjamin T JuliusDavid M Braun
Aug 28, 2020·Frontiers in Plant Science·Kayla Beam, José Trinidad Ascencio-Ibáñez
Feb 28, 2021·Physiologia Plantarum·Basavantraya N DevannaTilak R Sharma

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