Tomographic flow cytometry by digital holography

Light, Science & Applications
Francesco MerolaPietro Ferraro

Abstract

High-throughput single-cell analysis is a challenging task. Label-free tomographic phase microscopy is an excellent candidate to perform this task. However, in-line tomography is very difficult to implement in practice because it requires a complex set-up for rotating the sample and examining the cell along several directions. We demonstrate that by exploiting the random rolling of cells while they are flowing along a microfluidic channel, it is possible to obtain in-line phase-contrast tomography, if smart strategies for wavefront analysis are adopted. In fact, surprisingly, a priori knowledge of the three-dimensional position and orientation of rotating cells is no longer needed because this information can be completely retrieved through digital holography wavefront numerical analysis. This approach makes continuous-flow cytotomography suitable for practical operation in real-world, single-cell analysis and with a substantial simplification of the optical system; that is, no mechanical scanning or multi-direction probing is required. A demonstration is given for two completely different classes of biosamples: red blood cells and diatom algae. An accurate characterization of both types of cells is reported, despite their very...Continue Reading

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Citations

Aug 7, 2018·Journal of Biophotonics·Martina MugnanoPietro Ferraro
Oct 13, 2018·Journal of Biophotonics·Chenfei HuGabriel Popescu
Jan 22, 2019·Nature Communications·Daniel MidtvedtGavin D M Jeffries
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Aug 9, 2017·Nature Communications·Tan H NguyenGabriel Popescu
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Oct 28, 2019·Light, Science & Applications·Yair RivensonAydogan Ozcan
Sep 16, 2019·Journal of Biomedical Optics·Maciej TrusiakVicente Mico
Jul 10, 2020·Lab on a Chip·Akihiro IsozakiKeisuke Goda
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Nov 24, 2017·Lab on a Chip·Massimiliano M VillonePietro Ferraro
Apr 3, 2020·Journal of Biophotonics·Ziv ShemeshZeev Zalevsky
Aug 31, 2020·Light, Science & Applications·Xi ChenGabriel Popescu
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Aug 20, 2020·Scientific Reports·Maciej TrusiakKrzysztof Patorski
Jul 19, 2017·Biomedical Optics Express·Aihui SunCheng Liu

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Methods Mentioned

BETA
X-ray
imaging techniques
Chip

Software Mentioned

CUDA C platform
Nvidia

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