TonB protein appears to transduce energy by shuttling between the cytoplasmic membrane and the outer membrane in Escherichia coli

Molecular Microbiology
Tracy E Letain, Kathleen Postle

Abstract

The energy source for active transport of iron-siderophore complexes and vitamin B12 across the outer membrane in Gram-negative bacteria is the cytoplasmic membrane proton-motive force (pmf). TonB protein is required in this process to transduce cytoplasmic membrane energy to the outer membrane. In this study, Escherichia coli TonB was found to be distributed in sucrose density gradients approximately equally between the cytoplasmic membrane and the outer membrane fractions, while two proteins with which it is known to interact, ExbB and ExbD, as well as the NADH oxidase activity characteristic of the cytoplasmic membrane, were localized in the cytoplasmic membrane fraction. Neither the N-terminus of TonB nor the cytoplasmic membrane pmf, both of which are essential for TonB activity, were required for TonB to associate with the outer membrane. When the TonB C-terminus was absent, TonB was found associated with the cytoplasmic membrane, suggesting that the C-terminus was required for outer membrane association. When ExbB and ExbD, as well as their cross-talk-competent homologues ToIQ and ToIR, were absent, TonB was found associated with the outer membrane. TetA-TonB protein, which cannot interact with ExbB/D, was likewise found...Continue Reading

Citations

Apr 4, 2000·Current Opinion in Microbiology·C Wandersman, I Stojiljkovic
Mar 6, 1999·Current Opinion in Microbiology·P E Klebba, S M Newton
Feb 13, 2001·Molecular Microbiology·S S SeligerS M Payne
Jun 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Nicholas R SandovalRyan T Gill
Mar 26, 2010·The Journal of Biological Chemistry·Salete M NewtonPhillip E Klebba
Jul 6, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·David D ShultisMichael C Wiener
Oct 18, 2000·Infection and Immunity·S A ReevesS M Payne
Apr 12, 2012·Journal of Bacteriology·Anne A Ollis, Kathleen Postle
Apr 9, 2008·Journal of Bacteriology·Wallace A KasererPhillip E Klebba
May 8, 2007·Journal of Bacteriology·Hema Vakharia-RaoKathleen Postle
Oct 30, 2007·Journal of Bacteriology·Kerry K Brinkman, Ray A Larsen
May 10, 2001·Journal of Bacteriology·H KillmannV Braun
Aug 24, 2002·Journal of Bacteriology·Kiara G Held, Kathleen Postle
Oct 26, 2002·Journal of Bacteriology·Christopher D Kenney, Cynthia Nau Cornelissen
Mar 2, 2002·Journal of Bacteriology·Qixun Zhao, Keith Poole
Mar 2, 2002·Journal of Bacteriology·Penelope I HiggsKathleen Postle
Feb 4, 2003·Journal of Bacteriology·Alexandra R Mey, Shelley M Payne
Dec 24, 2010·MBio·Kathleen PostleCheryl D Swayne
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Eric CascalesDanièle Cavard
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Hiroshi Nikaido
Apr 2, 2011·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Karla D Krewulak, Hans J Vogel
Mar 28, 2013·Archaea : an International Microbiological Journal·Pallavee Srivastava, Meenal Kowshik
Aug 7, 2013·BMC Evolutionary Biology·Jian-Bo YuanJian-Hai Xiang
Jun 8, 2010·Proteome Science·Pradeep R DumpalaAttila Karsi
Dec 2, 2011·PloS One·Swapna BhatLawrence J Shimkets

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