Tonic NMDA receptor signalling shapes endosomal organisation in mammalian cells.

Scientific Reports
Oleg O Glebov

Abstract

Calcium signalling through NMDA-type glutamate receptors (NMDARs) plays a key role in synaptic plasticity in the central nervous system (CNS). NMDAR expression has also been detected in other tissues and aberrant glutamate signalling has been linked to cancer; however, the significance of NMDAR function outside of the CNS remains unclear. Here, I show that removal of extracellular calcium rapidly decreases the size of early endosomes in primary human fibroblasts. This effect can be mimicked by blockade of NMDA-type glutamate receptors but not voltage-gated calcium channels (VGCCs), and can also be observed in primary hippocampal neurons and Jurkat T cells. Conversely, in a breast cancer cell line MDA-MB-231 NMDAR blockade results in an increase in endosomal size and decrease in number. These findings reveal that calcium signalling via glutamate receptors controls the structure of the endosomal system and suggest that aberrations in NMDAR-regulated membrane trafficking may be associated with cancer.

References

Aug 1, 1974·Journal of Cellular Physiology·J Bard, M O Wright
Jan 8, 1998·The European Journal of Neuroscience·R TurecekL Vyklický
Jan 1, 2003·The Journal of Cell Biology·Alpha S Yap, Eva M Kovacs
Mar 5, 2004·Journal of Neurophysiology·Maxim VolgushevUlf T Eysel
Oct 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Andrzej StepulakChrysanthy Ikonomidou
Dec 13, 2005·Nature Cell Biology·Oleg O GlebovBenjamin J Nichols
Dec 20, 2005·The Journal of Membrane Biology·M Abdul, N Hoosein
Jul 31, 2007·Biochemical and Biophysical Research Communications·Gianluca MiglioGrazia Lombardi
Dec 18, 2007·Cell·David E Clapham
Jun 16, 2009·Histochemistry and Cell Biology·Andrzej StepulakChrysanthy Ikonomidou
Sep 29, 2009·Breast Cancer Research and Treatment·William G NorthLauna Lynch
Jul 13, 2011·Cold Spring Harbor Perspectives in Biology·William A Catterall
Jul 23, 2011·Genes & Cancer·Inbal Wortzel, Rony Seger
Aug 31, 2011·Cold Spring Harbor Perspectives in Biology·Claire J FearnleyMartin D Bootman
Feb 14, 2012·Current Opinion in Neurobiology·David L Hunt, Pablo E Castillo
Feb 18, 2012·Cold Spring Harbor Perspectives in Biology·Michael J Higley, Bernardo L Sabatini
May 21, 2013·Nature Reviews. Neuroscience·Pierre PaolettiQiang Zhou
Nov 5, 2013·Nature Neuroscience·Yaroslav S ErmolyukKirill E Volynski
Jan 21, 2014·Cellular and Molecular Life Sciences : CMLS·Marisa BriniErnesto Carafoli
Apr 23, 2014·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Stephen I DeutschAndrew D Benson
Dec 9, 2014·Nature Biotechnology·Christiaan KlijnZemin Zhang
Feb 21, 2016·Trends in Cell Biology·Eiji ShigetomiBaljit S Khakh
Feb 28, 2016·Current Opinion in Cell Biology·Roberto VillaseñorMarino Zerial
Sep 24, 2016·Journal of Alzheimer's Disease : JAD·Rui Wang, P Hemachandra Reddy
Oct 11, 2017·Toxicology in Vitro : an International Journal Published in Association with BIBRA·MingJie WangShaoJie Yue
Apr 24, 2018·Frontiers in Molecular Neuroscience·Marek LadislavLadislav Vyklicky

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Methods Mentioned

BETA
confocal microscopy

Software Mentioned

Prism
ImageJ
GraphPad

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