PMID: 9530877Apr 8, 1998Paper

Tonoplast intrinsic proteins from cauliflower (Brassica oleracea L. var. botrytis): immunological analysis, cDNA cloning and evidence for expression in meristematic tissues

Planta
F BarrieuF Marty

Abstract

The vacuolar membrane (tonoplast) of plant cells contains aquaporins, protein channels that facilitate the selective transport of water. These tonoplast intrinsic proteins (TIPs) of 23-29 kDa belong to the ancient major intrinsic protein (MIP) family. A monospecific polyclonal antiserum directed against a 26 kDa intrinsic protein from the tonoplast of meristematic cells from cauliflower (Brassica oleracea L. var. botrytis) was used to screen a cDNA library. Two distinct cDNAs have been isolated. Both clones, c26-1 and c26-2, encode closely related TIPs. The c26-1 insert, consisting of 933 bp upstream of the poly(A) tail, is a full-length cDNA with an open reading frame encoding a protein of 251 amino acids with a calculated M(r) of 25,500. The c26-2 insert is a 5' truncated cDNA. The two cDNAs share 90.5% sequence identity within their overlapping coding regions but only 35% sequence identity in the 3' untranslated regions, indicating that highly related TIP-encoding genes are expressed in meristematic cells. Although TIPs have previously been found in a variety of cell types, they have not been found in meristems. The derived amino acid sequences (BobTIP26-1 and BobTIP26-2, respectively) closely resemble the aquaporin gamma-TI...Continue Reading

Citations

Jun 15, 2007·Functional & Integrative Genomics·Kerrie L Forrest, Mrinal Bhave
Jul 9, 2011·Journal of Experimental Botany·Matthieu BesseWieland Fricke
May 12, 2000·Plant Physiology·H H KirchH J Bohnert
May 1, 2008·Annual Review of Plant Biology·Christophe MaurelVéronique Santoni
Sep 18, 2001·Journal of Experimental Botany·F SiefritzR Kaldenhoff
Jan 9, 2021·BMC Plant Biology·Paula Garcia-IbañezMicaela Carvajal

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