Tonoplast-localized Ca2+ pumps regulate Ca2+ signals during pattern-triggered immunity in Arabidopsis thaliana

Proceedings of the National Academy of Sciences of the United States of America
Richard HillearySimon Gilroy

Abstract

One of the major events of early plant immune responses is a rapid influx of Ca2+ into the cytosol following pathogen recognition. Indeed, changes in cytosolic Ca2+ are recognized as ubiquitous elements of cellular signaling networks and are thought to encode stimulus-specific information in their duration, amplitude, and frequency. Despite the wealth of observations showing that the bacterial elicitor peptide flg22 triggers Ca2+ transients, there remain limited data defining the molecular identities of Ca2+ transporters involved in shaping the cellular Ca2+ dynamics during the triggering of the defense response network. However, the autoinhibited Ca2+-ATPase (ACA) pumps that act to expel Ca2+ from the cytosol have been linked to these events, with knockouts in the vacuolar members of this family showing hypersensitive lesion-mimic phenotypes. We have therefore explored how the two tonoplast-localized pumps, ACA4 and ACA11, impact flg22-dependent Ca2+ signaling and related defense responses. The double-knockout aca4/11 exhibited increased basal Ca2+ levels and Ca2+ signals of higher amplitude than wild-type plants. Both the aberrant Ca2+ dynamics and associated defense-related phenotypes could be suppressed by growing the aca4/...Continue Reading

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Citations

Jan 18, 2021·Current Opinion in Plant Biology·Jong Hum KimSheng Yang He
Mar 30, 2021·The Plant Journal : for Cell and Molecular Biology·Pascal HolzheuUrsula Kummer
Jun 3, 2021·Cells·Huimin RenShenkui Liu
Aug 17, 2021·Plant Molecular Biology·Riya BasuSudip Chattopadhyay
Apr 28, 2021·Plant Physiology·Francesca ResentiniAlex Costa
Aug 11, 2021·Annual Review of Cell and Developmental Biology·Sheng Luan, Chao Wang

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Methods Mentioned

BETA
FRET
PCR

Software Mentioned

PSC Colocalization Plugin
FIJI

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