PMID: 9550027Jun 1, 1997Paper

Tooth discoloration by blood: an in vitro histochemical study

Endodontics & Dental Traumatology
P D MarinG S Heithersay

Abstract

An in vitro model, using a modification of a technique devised by Freccia & Peters, was developed to investigate tooth staining following pulpal haemorrhage. Samples of whole blood, erythrocytes, plasma and platelet concentrate and saline were individually placed in the pulp chambers of groups of five teeth and centrifuged twice daily for 25 min over a period of 3 consecutive days. This confirmed that the blood pigment responsible for the staining was found only in those samples containing erythrocytes. Teeth stained with packed red cells were then prepared for histological examination and subjected to four histochemical tests: (1) benzidine, (2) zinc leuco, (3) Perl's and (4) Turnbull Blue to analyse some of the biochemical changes following haemorrhage into the pulp chamber. These tests showed that, following haemolysis of erythrocytes within dentine, haemoglobin was found either intact or as one of the haematin molecules with no further breakdown of the haem structure and no evidence of any free ferric ions or haemosiderin.

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Citations

Nov 30, 2006·The American Journal of Forensic Medicine and Pathology·Carlo P CampobassoFrancesco Introna
Apr 29, 1998·Endodontics & Dental Traumatology·P D MarinT E Bridges
Oct 31, 2013·Clinical Oral Investigations·A NiklasG Schmalz
Mar 26, 2013·Journal of Endodontics·Daniel Felman, Peter Parashos
Mar 25, 2008·Journal of Endodontics·Gianluca PlotinoFrancesco Somma
May 16, 2007·Australian Dental Journal·K MaioloG S Heithersay
Nov 26, 2010·International Endodontic Journal·J K ParkG C Kim
May 25, 2012·International Endodontic Journal·H M A Ahmed, P V Abbott
Jun 4, 2015·European Journal of Dentistry·Da-A YunKyung-San Min
Apr 11, 2017·Indian Journal of Dental Research : Official Publication of Indian Society for Dental Research·Rashmi Keshav ChahandeDeepa A Thakur
Sep 1, 2017·Journal of Esthetic and Restorative Dentistry : Official Publication of the American Academy of Esthetic Dentistry ... [et Al.]·Melissa Andreia MarchesanFranklin García-Godoy
Jun 1, 1997·Endodontics & Dental Traumatology·S W DahlstromT E Bridges
Dec 6, 2003·Australian Endodontic Journal : the Journal of the Australian Society of Endodontology Inc·Geoffrey S Heithersay, Gawler Chambers
Jul 10, 2020·The Journal of Clinical Pediatric Dentistry·S ElbaharyH Azem
Apr 28, 2001·British Dental Journal·A Watts, M Addy
May 24, 2019·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Gideon Holan
Apr 1, 2017·Frontiers in Physiology·Gabriel HertigFranz E Weber
Aug 6, 2019·Journal of Oral Science·Irini FagogeniAlicja Nowicka
Nov 27, 2021·Journal of Veterinary Dentistry·Kristina FeiginChristopher Snyder
Dec 4, 2021·Journal of Esthetic and Restorative Dentistry : Official Publication of the American Academy of Esthetic Dentistry ... [et Al.]·Bruna GaidarjiLetícia Brandão Durand

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