Top-Down Analysis of Proteins in Low Charge States

Journal of the American Society for Mass Spectrometry
Aarti BashyalJennifer S Brodbelt

Abstract

The impact of charging methods on the dissociation behavior of intact proteins in low charge states is investigated using HCD and 193 nm UVPD. Low charge states are produced for seven different proteins using the following four different methods: (1) proton transfer reactions of ions in high charge states generated from conventional denaturing solutions; (2) ESI of proteins in solutions of high ionic strength to enhance retention of folded native-like conformations; (3) ESI of proteins in high pH solutions to limit protonation; and (4) ESI of carbamylated proteins. Comparison of sequence coverages, degree of preferential cleavages, and types and distribution of fragment ions reveals a number of differences in the fragmentation patterns depending on the method used to generate the ions. More notable differences in these metrics are observed upon HCD than upon UVPD. The fragmentation caused by HCD is influenced more significantly by the presence/absence of mobile protons, a factor that modulates the degree of preferential cleavages and net sequence coverages. Carbamylation of the lysines and the N-terminus of the proteins alters the proton mobility by reducing the number of proton-sequestering, highly basic sites as evidenced by ...Continue Reading

References

Nov 10, 2000·Journal of the American Society for Mass Spectrometry·A T IavaroneE R Williams
Feb 17, 2001·Journal of Mass Spectrometry : JMS·V H WysockiL A Breci
Nov 25, 2003·Analytical Chemistry·Anthony T Iavarone, Evan R Williams
Mar 5, 2004·Journal of Proteome Research·Sharon J PitteriScott A McLuckey
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·John E P SykaDonald F Hunt
Aug 28, 2007·Nature Methods·Jesper V OlsenMatthias Mann
Jun 2, 2009·Journal of the American Society for Mass Spectrometry·Casey J KrusemarkLloyd M Smith
Feb 18, 2010·International Journal of Mass Spectrometry·Chamnongsak ChanthamontriScott A McLuckey
Mar 23, 2010·Journal of the American Society for Mass Spectrometry·Jennifer S CobbJeffrey N Agar
Jan 17, 2012·Proteomics·Min-Sik Kim, Akhilesh Pandey
Feb 28, 2013·Nature Methods·Lloyd M SmithUNKNOWN Consortium for Top Down Proteomics
May 24, 2013·Journal of the American Chemical Society·Jared B ShawJennifer S Brodbelt
Jan 23, 2014·Analytical Chemistry·Joe R CannonJennifer S Brodbelt
Feb 22, 2014·Biochemical and Biophysical Research Communications·Adam D CathermanNeil L Kelleher
Mar 25, 2014·Journal of the American Society for Mass Spectrometry·Huilin ShiDavid E Clemmer
Nov 26, 2015·The Analyst·Lindsay J Morrison, Jennifer S Brodbelt
Dec 3, 2015·Analytical Chemistry·Jennifer S Brodbelt
Jan 23, 2016·Journal of Proteome Research·Kenneth Robert DurbinNeil L Kelleher
Apr 8, 2016·Journal of the American Society for Mass Spectrometry·Rachel R Ogorzalek Loo, Joseph A Loo
May 22, 2016·Journal of the American Society for Mass Spectrometry·Lindsay J MorrisonJennifer S Brodbelt
Jun 17, 2016·Annual Review of Analytical Chemistry·Timothy K TobyNeil L Kelleher
Jul 12, 2016·Journal of the American Chemical Society·Kenneth J LaszloMatthew F Bush
Aug 9, 2016·Journal of the American Society for Mass Spectrometry·Frederik LermyteFrank Sobott
Oct 14, 2016·Journal of the American Society for Mass Spectrometry·Anna C SusaEvan R Williams
Feb 23, 2017·Journal of the American Society for Mass Spectrometry·Kenneth J LaszloMatthew F Bush
Apr 5, 2017·Journal of the American Society for Mass Spectrometry·Nicole A HaverlandNeil L Kelleher
Apr 5, 2017·Journal of the American Society for Mass Spectrometry·Sylvester M GreerJennifer S Brodbelt
Jul 14, 2017·Journal of the American Society for Mass Spectrometry·Ryan R Julian
Jul 16, 2017·Journal of the American Society for Mass Spectrometry·Lars Konermann
Nov 28, 2017·Analytical Chemistry·Nicholas M Riley, Joshua J Coon
Jan 19, 2018·Journal of Proteome Research·Sylvester M Greer, Jennifer S Brodbelt
Apr 3, 2018·Analytical Chemistry·James D SandersJennifer S Brodbelt
Apr 8, 2018·Journal of the American Society for Mass Spectrometry·Jake RosenbergJennifer S Brodbelt

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Citations

Jan 1, 2020·Molecular & Cellular Proteomics : MCP·Luca FornelliNeil L Kelleher
Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Luca FornelliNeil L Kelleher
Mar 24, 2021·Journal of the American Society for Mass Spectrometry·Lei LuLloyd M Smith
Jul 28, 2019·Journal of the American Society for Mass Spectrometry·Frederik LermyteJoanna F Collingwood
Nov 26, 2020·Journal of the American Society for Mass Spectrometry·Frederik LermytePeter B O'Connor
Dec 19, 2019·Chemical Reviews·Jennifer S BrodbeltInês Santos
Jun 17, 2020·The Journal of Physical Chemistry. a·Kailin ZhangXianglei Kong
Nov 7, 2019·Analytical Chemistry·David Foreman, Scott A McLuckey
Oct 30, 2021·Journal of the American Society for Mass Spectrometry·Luis A MaciasJennifer S Brodbelt
Dec 28, 2021·Journal of the American Society for Mass Spectrometry·Yuan LinLissa C Anderson

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