Abstract
We report a scalable, uniform, and sensitive top-down fabricated indium oxide (In2O3) nanoribbon biosensor platform with integrated on-chip gate electrodes using two photolithographic masks. The purpose of this on-chip gate electrode is to control the operational point of the sensor during biomolecular detection replacing the cumbersome external Ag/AgCl electrode. It exhibits excellent capability in gating transistors in an aqueous condition and high stability during the sensing experiment, which is similar to the Ag/AgCl electrode. Its compactness increases the portability and pushes this platform toward a practical use. To demonstrate its capability for detection of biomolecules, we combine this platform with the electronic enzyme-linked immunosorbent assay (ELISA) technique to amplify the signal and to bypass limitation of the Debye screening effect from high salt concentration of physiological samples. Troponin I, a cardiac marker for diagnosis of acute myocardial infarction (AMI), was selected as the target molecule in this study. The In2O3 nanoribbon device offers a high response of 30% toward 0.1 pg ml-1 troponin I concentration and a lower detection limit than that of the commercial ELISA kit on the market by five order...Continue Reading
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