Top down metabolic control analysis

Journal of Theoretical Biology
M D Brand

Abstract

In the top down approach to metabolic control analysis, reactions are grouped together into a few large blocks connected by a small number of intermediates. Control is analysed by adding inhibitor or activators that work within single blocks and measuring intermediates and fluxes, or by adding new branches and measuring changes in flux. The approach is experimentally and conceptually simple, and provides a complete but coarse description of control in complex metabolic systems that are not easily accessible using other approaches. This paper gives an overview of the method, its equations and their derivations. It discusses the ways the approach has been used in organelles and cells to identify the sites of action of effectors, to measure the distribution of control by blocks of reactions, and to measure regulation by effectors.

Citations

Sep 1, 1999·European Journal of Biochemistry·E K Ainscow, M D Brand
Mar 6, 2008·Annual Review of Biochemistry·Michael P Murphy, Linda Partridge
Sep 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Boris N KholodenkoJan B Hoek
Jul 6, 2011·The Biochemical Journal·Martin D Brand, David G Nicholls
Oct 7, 2003·Annual Review of Biomedical Engineering·Martin L Yarmush, Scott Banta
Jan 10, 2017·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Yanfei Zhang, José L Avalos
Apr 1, 1999·Biotechnology and Bioengineering·B N KholodenkoJ Schwaber
Nov 7, 2003·American Journal of Physiology. Cell Physiology·Jeffrey I MesserWayne T Willis
Dec 3, 1999·European Journal of Biochemistry·E K Ainscow, M D Brand
Jul 6, 2000·FEBS Letters·J S Hofmeyr, A Cornish-Bowden
Feb 17, 2015·Biotechnology Progress·Luis AcerenzaFernando Ortega
Nov 25, 2004·American Journal of Physiology. Endocrinology and Metabolism·Kevork HagopianJon J Ramsey
Oct 23, 2010·Biotechnology Progress·Korkut UygunYinlun Huang
Oct 14, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jason C L BrownJames F Staples
Aug 8, 2013·Aging Cell·Gilles GouspillouPhilippe Diolez
Feb 9, 2017·Frontiers in Oncology·Eduardo N Maldonado
Dec 2, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S Krauss, M D Brand
Dec 10, 2003·American Journal of Physiology. Endocrinology and Metabolism·Jon J RamseyMary-Ellen Harper
Jan 16, 2007·The FEBS Journal·Luis Acerenza, Fernando Ortega
Jan 17, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Tammie BishopMartin D Brand
Sep 13, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·A J Lambert, B J Merry
Sep 15, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Philippe DiolezJean-Michel Franconi
Oct 4, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Guillaume CalmettesPhilippe Diolez
Sep 3, 2005·The Biochemical Journal·Charles Affourtit, Martin D Brand
Jul 2, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Véronique Deschodt-ArsacPhilippe Diolez
Jan 26, 2016·Medicine and Science in Sports and Exercise·Wayne T WillisBrian Glancy

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