Top-Down Proteomics Enables Comparative Analysis of Brain Proteoforms Between Mouse Strains

Analytical Chemistry
Roderick G DavisNeil L Kelleher

Abstract

Over the past decade, advances in mass spectrometry-based proteomics have accelerated brain proteome research aimed at studying the expression, dynamic modification, interaction and function of proteins in the nervous system that are associated with physiological and behavioral processes. With the latest hardware and software improvements in top-down mass spectrometry, the technology has expanded from mere protein profiling to high-throughput identification and quantification of intact proteoforms. Murine systems are broadly used as models to study human diseases. Neuroscientists specifically study the mouse brain from inbred strains to help understand how strain-specific genotype and phenotype affect development, functioning, and disease progression. This work describes the first application of label-free quantitative top-down proteomics to the analysis of the mouse brain proteome. Operating in discovery mode, we determined physiochemical differences in brain tissue from four healthy inbred strains, C57BL/6J, DBA/2J, FVB/NJ, and BALB/cByJ, after probing their intact proteome in the 3.5-30 kDa mass range. We also disseminate these findings using a new tool for top-down proteomics, TDViewer and cataloged them in a newly establis...Continue Reading

References

May 1, 1996·Trends in Neurosciences·J N Crawley
May 20, 1997·European Journal of Pharmacology·A J RobertsL H Gold
Jul 1, 1997·Pharmacology, Biochemistry, and Behavior·V DerocheL H Gold
Dec 30, 1999·Nature Genetics·J A BeckE M Fisher
Feb 6, 2002·Genome Research·Andrew S Peterson
Mar 26, 2002·Nature Genetics·Joachim KloseHans Lehrach
Aug 20, 2004·BMC Genomics·Rolf TurkJohan T den Dunnen
Sep 3, 2004·Genome Research·Petko M PetkovMichael V Wiles
Apr 19, 2005·Nature Biotechnology·Yasushi IshihamaYoshiya Oda
Jul 13, 2005·Molecular & Cellular Proteomics : MCP·Alexey I Nesvizhskii, Ruedi Aebersold
Dec 13, 2005·Alcoholism, Clinical and Experimental Research·Yuri A BlednovJohn C Crabbe
Oct 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Douglas WahlstenJohn C Crabbe
Jul 31, 2007·Nature Genetics·Hyuna YangFernando Pardo-Manuel de Villena
Apr 29, 2008·Brain Research Reviews·Teresa Cunha-OliveiraCatarina R Oliveira
Mar 20, 2009·The Journal of Investigative Dermatology·Robert H RiceJohn P Sundberg
Aug 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·John C PriceSina Ghaemmaghami
Oct 20, 2010·Proceedings of the National Academy of Sciences of the United States of America·John A MorrisAllan R Jones
Jun 23, 2011·PLoS Genetics·Anatole GhazalpourAldons J Lusis
Sep 17, 2011·Nature·Thomas M KeaneDavid J Adams
Oct 10, 2012·Nature Reviews. Genetics·Jonathan Flint, Eleazar Eskin
May 9, 2014·Analytical Chemistry·Ioanna NtaiNeil L Kelleher
Jun 13, 2014·Journal of Proteome Research·Richard D LeDucNeil L Kelleher
Nov 3, 2015·Nature Neuroscience·Kirti SharmaMikael Simons
Feb 6, 2017·Molecular & Cellular Proteomics : MCP·Sung Yun JungJun Qin
May 17, 2017·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·T K TobyJ Levitsky

❮ Previous
Next ❯

Citations

Jan 17, 2019·Molecular & Cellular Proteomics : MCP·Richard D LeDucNeil L Kelleher
May 3, 2019·Proteomics·Leah V SchafferLloyd M Smith
Jan 28, 2021·International Journal of Molecular Sciences·Aneeqa NoorInga Zerr
Apr 1, 2019·Molecular & Cellular Proteomics : MCP·Richard D LeDucNeil L Kelleher
Apr 16, 2021·Journal of Proteome Research·James M FulcherVladislav A Petyuk
May 30, 2021·NPJ Systems Biology and Applications·Yassene MohammedChristoph H Borchers
Aug 13, 2021·Frontiers in Cell and Developmental Biology·Ni PanCuihong Wan
Nov 2, 2019·Analytical Chemistry·Luis A MaciasJennifer S Brodbelt
Apr 17, 2019·Journal of Proteome Research·Zheyi LiuFangjun Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

CZI Human Cell Atlas Seed Network

The aim of the Human Cell Atlas (HCA) is to build reference maps of all human cells in order to enhance our understanding of health and disease. The Seed Networks for the HCA project aims to bring together collaborators with different areas of expertise in order to facilitate the development of the HCA. Find the latest research from members of the HCA Seed Networks here.