Topical Antibiotic Elution in a Collagen-Rich Hydrogel Successfully Inhibits Bacterial Growth and Biofilm Formation In Vitro.

Antimicrobial Agents and Chemotherapy
Jung Gi MinPaige M Fox

Abstract

Chronic wounds are a prominent concern, accounting for $25 billion of health care costs annually. Biofilms have been implicated in delayed wound closure, but they are susceptible to developing antibiotic resistance and treatment options continue to be limited. A novel collagen-rich hydrogel derived from human extracellular matrix presents an avenue for treating chronic wounds by providing appropriate extracellular proteins for healing and promoting neovascularization. Using the hydrogel as a delivery system for localized secretion of a therapeutic dosage of antibiotics presents an attractive means of maximizing delivery while minimizing systemic side effects. We hypothesize that the hydrogel can provide controlled elution of antibiotics leading to inhibition of bacterial growth and disruption of biofilm formation. The rate of antibiotic elution from the collagen-rich hydrogel and the efficacy of biofilm disruption was assessed with Pseudomonas aeruginosa Bacterial growth inhibition, biofilm disruption, and mammalian cell cytotoxicity were quantified using in vitro models. The antibiotic-loaded hydrogel showed sustained release of antibiotics for up to 24 h at therapeutic levels. The treatment inhibited bacterial growth and disr...Continue Reading

References

Mar 9, 2000·Journal of Pharmaceutical and Biomedical Analysis·P FrutosG Frutos
Nov 19, 2003·Advanced Drug Delivery Reviews·Donald G Wallace, Joel Rosenblatt
Dec 13, 2005·Biomaterials·Peng Wu, David W Grainger
Apr 1, 2006·BMJ : British Medical Journal·Vanessa JonesKeith G Harding
Dec 19, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Garth A JamesPhilip S Stewart
Jan 29, 2008·Journal of the American Academy of Dermatology·Margaret A FonderAdam J Mamelak
May 30, 2008·Journal of Clinical Microbiology·Klaus Kirketerp-MøllerThomas Bjarnsholt
Mar 11, 2010·Journal of Wound Care·R D WolcottS E Dowd
Aug 25, 2010·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Ge ZhaoJohn E Olerud
Dec 28, 2010·Colloids and Surfaces. B, Biointerfaces·Eleonora MarsichSergio Paoletti
Feb 11, 2011·Journal of Visualized Experiments : JoVE·George A O'Toole
Apr 13, 2011·International Journal of Oral Science·Niels HøibyThomas Bjarnsholt
Apr 13, 2011·International Journal of Oral Science·Liang YangZhi-jun Song
Apr 27, 2011·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Mustafa FazliTim Tolker-Nielsen
Jun 15, 2011·Antimicrobial Agents and Chemotherapy·Wang HengzhuangNiels Høiby
Nov 29, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Bin JiangEric M Brey
Dec 18, 2012·Tissue Engineering. Part a·Shane BrowneAbhay Pandit
Jul 6, 2013·Trends in Microbiology·Thomas BjarnsholtNiels Høiby
Aug 15, 2013·Bioengineered·Oded ShoseyovFrida Grynspan
Oct 1, 2013·International Journal of Pharmaceutics·Baljit SinghAbhishek Dhiman
Feb 15, 2014·Advances in Wound Care·Ge ZhaoJohn E Olerud
Apr 23, 2014·Advanced Drug Delivery Reviews·Nicholas D StebbinsKathryn E Uhrich
Dec 17, 2014·International Journal of Oral Science·Hong WuZhi-Jun Song
Mar 10, 2015·Expert Review of Anti-infective Therapy·Raffaele SerraStefano de Franciscis
Jun 1, 2017·Biotechnology Advances·Sheva NaahidiP Chen
Aug 5, 2017·Materials·Carmen M González-HenríquezJuan Rodriguez-Hernandez
Feb 21, 2018·The International Journal of Lower Extremity Wounds·Konstantinos MarkakisFrank L Bowling
Apr 27, 2018·International Journal of Nanomedicine·Kerong YangJincheng Wang
Feb 13, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yukitoshi KaizawaPaige M Fox
Oct 9, 2019·Plastic and Reconstructive Surgery. Global Open·Ibrahim KhansaJeffrey E Janis
Aug 7, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Gisela Solange AlvarezMartin Federico Desimone

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Citations

Jun 3, 2021·Bioengineering·Shomita S Mathew-SteinerChandan K Sen

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