Topical Application of Culture-Expanded CD34+ Umbilical Cord Blood Cells from Frozen Units Accelerates Healing of Diabetic Skin Wounds in Mice

Stem Cells Translational Medicine
Jennifer WhiteleyIan M Rogers

Abstract

Chronic and nonhealing wounds are constant health issues facing patients with type 2 diabetes. As the incidence of type 2 diabetes mellitus (T2DM) increases, the incidence of chronic wounds and amputations will rise. T2DM is associated with peripheral arterial occlusive disease, which leads to the development of nonhealing skin ulcers after minor trauma. Patients develop severe pain limiting their mobility and ability to work and take care of themselves, thus putting a significant burden on the family and society. CD34+ cells from umbilical cord blood (UCB) grown in fibroblast growth factor-4 (FGF-4), stem cell factor, and Flt3-ligand produced a population of cells that have the ability to proliferate and develop properties enabling them to enhance tissue regeneration. The goal of this study was to assess in vitro cultured CD34+ cells in a setting where they would eventually be rejected so we could isolate paracrine signaling mediated therapeutic effect from the therapeutic effect due to engraftment and differentiation. To achieve this, we used db/db mice as a model for diabetic skin ulcers. Here, we report that in vitro cultured UCB CD34+ cells from frozen units can accelerate wound healing and resulted in the regeneration of ...Continue Reading

References

Oct 17, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A F LaplanteL Germain
Sep 2, 1999·The New England Journal of Medicine·A J Singer, R A Clark
Jun 10, 2000·British Journal of Haematology·A EricesJ J Minguell
Jun 24, 2000·Molecular and Cellular Biology·S M WojcikD R Roop
Aug 23, 2000·The Journal of Clinical Investigation·G C SchattemanB A Christy
Apr 23, 2003·Journal of the American Society of Nephrology : JASN·Roger M Mason, Nadia Abdel Wahab
Aug 2, 2003·Journal of Vascular Research·E Sivan-LoukianovaG C Schatteman
May 3, 2005·Journal of the American College of Cardiology·Gian Paolo FadiniAngelo Avogaro
Dec 13, 2005·American Journal of Physiology. Heart and Circulatory Physiology·David A KlibanskyJay M Edelberg
Apr 4, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sophia S CheonBenjamin A Alman
Apr 8, 2006·Journal of Hypertension. Supplement : Official Journal of the International Society of Hypertension·Roland E Schmieder
Apr 26, 2006·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Marcie R FinneyMary J Laughlin
Jul 22, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Gian Paolo FadiniAngelo Avogaro
May 11, 2007·Journal of Molecular and Cellular Cardiology·B W A van der StrateM J A van Luyn
Jun 1, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Elisabeth H JavazonAlan W Flake
Aug 11, 2007·Mechanisms of Ageing and Development·Shinobu SugiharaIchiro Hisatome
Nov 1, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Joseph MichaelsGeoffrey C Gurtner
Nov 22, 2007·Matrix Biology : Journal of the International Society for Matrix Biology·Nadav SegalJonathan A Garlick
Jul 22, 2008·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Ian M RogersRobert F Casper
May 18, 2010·Scandinavian Journal of Clinical and Laboratory Investigation·Qiang TanWansong Yang
Jul 12, 2011·Expert Reviews in Molecular Medicine·Timothy J Koh, Luisa Ann DiPietro
Nov 19, 2011·Plastic and Reconstructive Surgery·Cheng PengLifei Guo
Dec 14, 2011·Interactive Cardiovascular and Thoracic Surgery·Mohamed A ElsharawySahar Greish
Mar 7, 2012·North American Journal of Medical Sciences·Ana Maria Abreu-Velez, Michael S Howard
Apr 13, 2012·The European Respiratory Journal·Christopher D BakerSteven H Abman
Jan 25, 2014·Journal of Innate Immunity·Ilknur SenyürekBirgit Schittek
Mar 1, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Vivian TamKenneth M C Cheung
Jul 30, 2014·Advances in Therapy·Dimitrios BaltzisAristidis Veves
Oct 14, 2014·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·D TokiP J O'Connell
May 24, 2015·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·M KallioE Tukiainen
Apr 14, 2016·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Shao-Wei YangXiao-Bing Fu

❮ Previous
Next ❯

Citations

Mar 26, 2019·Advanced Materials·Benjamin R Freedman, David J Mooney
Jul 28, 2019·Experimental Dermatology·Shadi KhalilArturo P Saavedra
Mar 16, 2021·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Pratheesh D MankuzhyTaru Guttula Sharma

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
biopsy
amputation

Software Mentioned

Nanozoom Digital Pathology view ( NDP . view2 )
Prism Graph Pad
Image J

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.