Topical Application of Insulin Accelerates Vessel Maturation of Wounds by Regulating Angiopoietin-1 in Diabetic Mice

The International Journal of Lower Extremity Wounds
Chaofei LiXiong Zhang

Abstract

Reestablishment of the structural and functional microvasculature would be beneficial to promote healing of diabetic wounds. We explored the role of insulin application on microvascular maturation of diabetic wounds to determine whether it is associated with insulin-induced wound healing. We adopted the multiple injections of streptozotocin (STZ) to establish a diabetic animal model. The effect of insulin on microvessel formation, especially the effect of insulin on microvascular maturation was observed by transmission electron microscopy and laser scanning confocal microscopy. The pivotal protein regulated by insulin during healing processes was explored by tropical application neutralizing antibodies to these proteins; the specific protein was further confirmed using immunoblotting. On days 7 and 11, the blood vessel in insulin-treated wounds was surrounded by more α-smooth muscle actin (α-SMA) expressing cells. The blockage of angiopoietin-1 (Ang-1), but not angiopoietin-2 (Ang-2) or platelet-derived growth factor-B (PDGF-B), resulted in reduced maturation of newly formed blood vessels despite the presence of insulin in vivo. Further analysis showed that insulin induced an increased expression of Ang-1. The blood vessels in ...Continue Reading

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Citations

Sep 23, 2016·Archives of Dermatological Research·T EmanuelliE Carvalho
Jul 4, 2017·International Journal of Molecular Sciences·Uzoagu A Okonkwo, Luisa A DiPietro

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Methods Mentioned

BETA
transmission
confocal microscopy
enzyme-linked immunosorbent assay
ELISA
protein assay
Infrared Imaging
transmission electron microscopy

Software Mentioned

Image J
Metamorph
Photoshop

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