Topical effect of various agents on gelatinase activity in the tear film of normal dogs

Veterinary Ophthalmology
Simon CoutureMichel Carrier

Abstract

To evaluate the topical effect of various agents, currently used in the treatment of melting ulcers, on gelatinase activity present in the tear film of normal dogs. Eight normal adult beagles. Each animal received the following agents: cyclosporine A 1%, N-acetylcysteine 10%, ciprofloxacin 0.3%, ethylenediaminetetraacetic acid (EDTA) 1%, doxycycline 0.001%, polysulfated glycosaminoglycans (PSGAG) 5%, autoserum, and artificial tears during a 48-h period following a Latin square design. Tear samples were collected with micro-capillary pipettes following a corneal surface irrigation of each eye with sterile saline on four different occasions. Basal and total gelatinase activities were evaluated by optical density after processing in a commercial gelatinase activity assay. From the optical density ratio, a semi-quantitative measure of gelatinase activity was obtained. Basal and total activities were measured in all samples. The lowest total gelatinase activity, representing a percent decline in the enzyme activity although not significant, was observed 1 h after the last treatment in seven out of the eight ophthalmic agents; EDTA (68%), ciprofloxacin (76%), cyclosporine A (68%), doxycycline (47%), artificial tears (26%), PSGAG (25%...Continue Reading

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Citations

Dec 3, 2008·Veterinary Ophthalmology·Amy BakerDennis E Brooks
Aug 25, 2016·Journal of the American Veterinary Medical Association·Sean P CollinsRalph E Hamor
Aug 1, 2007·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·L F M BarrosR Belfort Jr
Jul 31, 2021·Veterinary Medicine International·Jéssica Fontes VelosoRenata Santiago Alberto Carlos

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