PMID: 11336354May 5, 2001Paper

Topical gene transfer into rat skin using electroporation

Pharmaceutical Research
N DujardinV Préat

Abstract

To investigate whether electroporation can be used for topical gene delivery and for DNA expression in rat keratinocytes. The localization of a fluorescent-labelled plasmid and the expression of a reporter gene (pEGFP-N1) coding for Green Fluorescent Protein (GFP) in stripped skin were assessed by Confocal Laser Scanning Microscopy (CLSM). The plasmid penetrated into the epidermis within minutes after electroporation and entered the keratinocyte cytoplasm within hours. A localized expression of GFP was observed for at least 7 days in the epidermis. Skin viability was not compromised by electroporation. Electroporation enhances the delivery, and hence the expression, of topically applied plasmid DNA on the skin. It could be a promising alternative method to administer DNA, particularly for DNA vaccines, in the skin in vivo.

Citations

Nov 29, 2002·Gene Therapy·T Niidome, L Huang
Jul 21, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Colman K ByrnesJohn W Harmon
Mar 7, 2006·Ultrastructural Pathology·V Vicente OrtegaF Calderón Rubiales
Jun 7, 2014·Annual Review of Biomedical Engineering·Martin L YarmushDamijan Miklavčič
Jun 1, 2017·Pleura and Peritoneum·Margo SteuperaertWim Ceelen
Mar 17, 2004·Lasers in Surgery and Medicine·Makoto OguraMinoru Obara
Oct 6, 2007·Expert Opinion on Biological Therapy·Julianna LisziewiczFranco Lori
Apr 9, 2005·World Journal of Surgery·Mark FergusonJohn W Harmon
Jul 15, 2005·Skin Pharmacology and Physiology·M J Choi, H I Maibach
May 17, 2005·The Journal of Gene Medicine·Ildiko BadeaMarianna Foldvari
Oct 21, 2009·Human Gene Therapy·Richard HellerMark J Jaroszeski
Jul 25, 2021·Bioelectrochemistry·Reginald M AtkinsMark J Jaroszeski
Sep 28, 2021·ACS Applied Materials & Interfaces·Ivana SremačkiAnton Nikiforov

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