Topical glucocorticoids modulate the lesion interface after cerebral cortical stab wounds in adult rats

Glia
M S Li, S David

Abstract

A lesion interface, consisting of a glia limitans lined by a laminin-rich basal lamina and leptomeningeal cells, forms within 2-3 weeks after penetrating wounds to the adult mammalian central nervous system (CNS). This interface prevents the growth of axons across the lesion. We have examined the effects of topically applied steroids on the formation of such an interface after stab wounds to the adult rat cerebral cortex. Immediately after lesioning, the surface of cortex in the region of the wound was treated with a topical application of either 0.1% halcinonide or 0.05% betamethasone dipropionate or their respective placebos. Cryostat sections through the lesioned area were obtained 3 weeks later and assessed by immunofluorescence. Steroid treatment attenuated all components of the lesion. The continuous anti-laminin labeling along the lesion in untreated rats became patchy after steroid treatment. The number of leptomeningeal cells that infiltrated into the wound was reduced in the laminin-negative regions in steroid-treated rats. In addition, astrocytic processes in the laminin-negative regions after steroid treatment were loosely arranged, compared with the tightly packed parallel processes forming the glia limitans in lam...Continue Reading

Citations

May 16, 2002·Experimental Neurology·F De WinterJ Verhaagen
Jan 27, 2004·Biomaterials·Young-Tae KimPatrick A Tresco
Sep 14, 1999·Brain Research Bulletin·J W Fawcett, R A Asher
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Sep 8, 2005·Tissue Engineering·Jennifer F WalshPatrick A Tresco
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Jun 25, 2014·Neuroscience Bulletin·Dingding ShenXiaosong Gu
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Jan 3, 2013·Trends in Molecular Medicine·Ravid Shechter, Michal Schwartz
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