PMID: 11904462Mar 21, 2002Paper

Topical mitomycin C and radiation induce conjunctival DNA-polyploidy

Analytical Cellular Pathology : the Journal of the European Society for Analytical Cellular Pathology
O CartsburgA Böcking

Abstract

Atypical cell changes often occur following treatment of premalignant or malignant conjunctival neoplasias with topical mitomycin C (MMC) and/or radiation. These reactive, non-neoplastic alterations of the conjunctival epithelium can be a differential diagnostic problem. Our aim was to investigate changes in the nuclear DNA-distribution of conjunctival epithelial cells after MMC- and radiation therapy by DNA-image-cytometry. Conjunctival brush smears were obtained from 13 patients (13 eyes) with squamous cell carcinomas and six patients (6 eyes) with conjunctival malignant melanomas in situ before, during and after treatment. The patients were treated with MMC-drops (0.02% or 0.04%) alone (n=12), with radiation therapy (n=3) or both (n=4). At first, the obtained brush smears were evaluated by cytology. Secondly, after Feulgen restaining, the DNA content of reactively changed cells was determined using the AutoCyte-QUIC-DNA workstation. We observed euploid DNA-polyploidy and cytomorphological changes in all patients (19/19). We considered these alterations as reactive to treatment. Four patients showed their greatest DNA-stemline at 4c and 15 patients at 8c. This effect was observed during and following MMC-drops and/or radiatio...Continue Reading

Citations

Aug 8, 2009·American Journal of Ophthalmology·Ekaterina A SemenovaSteven A McCormick
Mar 1, 2005·American Journal of Ophthalmology·Jacob Pe'er, Joseph Frucht-Pery
Mar 2, 2013·The Cochrane Database of Systematic Reviews·Stephen Gichuhi, James H Irlam
Jul 16, 2015·Asia-Pacific Journal of Ophthalmology·Rakhi KusumeshRajiv Kumar
Mar 18, 2021·Seminars in Cancer Biology·Halina WasClaudine Kieda

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