Topical Mitomycin-C enhances subbasal nerve regeneration and reduces erosion frequency in the debridement wounded mouse cornea.

Experimental Eye Research
Sonali Pal-GhoshMary Ann Stepp

Abstract

Corneal epithelial basement membrane dystrophies and superficial injuries caused by scratches can lead to recurrent corneal erosion syndrome (RCES). Patients and animals with reduced corneal sensory nerve innervation can also develop recurrent erosions. Multiple wild-type mouse strains will spontaneously develop recurrent corneal erosions after single 1.5 mm debridement wounds. Here we show that this wound is accompanied by an increase in corneal epithelial cell proliferation after wound closure but without a commensurate increase in corneal epithelial thickness. We investigated whether excess corneal epithelial cell proliferation contributes to erosion formation. We found that topical application of Mitomycin C (MMC), a drug used clinically to improve healing after glaucoma and refractive surgery, reduces erosion frequency, enhances subbasal axon density to levels seen in unwounded corneas, and prevents excess epithelial cell proliferation after debridement wounding. These results suggest that topically applied MMC, which successfully reduces corneal haze and scarring after PRK, may also function to enhance subbasal nerve regeneration and epithelial adhesion when used to treat RCES.

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Citations

Feb 16, 2019·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·A Sue MenkoMary Ann Stepp
Sep 29, 2019·Investigative Ophthalmology & Visual Science·Xiaowen Lu, Mitchell A Watsky
Jul 10, 2016·Drug Delivery and Translational Research·Sonia Reimondez-TroitiñoMaría J Alonso
Dec 6, 2018·International Journal of Molecular Sciences·Mary Ann SteppCintia S de Paiva
Jul 28, 2020·Molecular and Cellular Biochemistry·Guadalupe Martínez-ChacónAlberto Ballestín
May 30, 2021·Translational Research : the Journal of Laboratory and Clinical Medicine·Mary Ann Stepp, A Sue Menko

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