Topical transplantation of mesenchymal stem cells accelerates gastric ulcer healing in rats

American Journal of Physiology. Gastrointestinal and Liver Physiology
Yujiro HayashiSunao Kawano

Abstract

Mesenchymal stem cells (MSCs), a subpopulation of adult somatic stem cells, are an attractive stem cell source in regenerative medicine because of their multipotentiality. We examined the effects of MSC transplantation on gastric ulcer healing. Putative MSCs, isolated from bone marrow aspirates of male rats by dish adherence and expanded in culture, were characterized by flow cytometry and reverse transcription-polymerase chain reaction. Gastric ulcers were induced by serosal application of acetic acid on the anterior wall of the stomach in female rats. Either MSCs (labeled with PKH67; 1x10(7) cells) or vehicle was injected into the gastric wall surrounding the ulcer. The healing process of the ulcer and the influence of anti-vascular endothelial growth factor (VEGF) antibody were examined. CD29-positive, CD90-positive, CD34-negative, and CD45-negative MSCs expressed mRNAs for VEGF and hepatocyte growth factor (HGF). The MSCs were transplantable to the gastric tissue surrounding the ulcer, where a majority of the engrafted cells were positive for vimentin. The transplantation significantly accelerated gastric ulcer healing compared with controls. The engrafted MSCs also expressed VEGF and HGF. Administration of anti-VEGF neutra...Continue Reading

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Citations

Jan 30, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Takuya NaritaKen Suzuki
Dec 29, 2010·Stem Cells and Development·Jacobo Trebol LopezDamián García-Olmo
Jun 17, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Nobuko TanoKen Suzuki
Nov 3, 2012·The Journal of Surgical Research·Yu SaitoMichihito Asanoma
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Oct 1, 2011·The Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi·Myung Shin Jeon, Soon Sun Hong
Aug 4, 2020·World Journal of Stem Cells·Yang Gao, Shi-Zhu Jin
Sep 28, 2021·Stem Cell Research & Therapy·Mahshid SalehAmir Abbas Vaezi

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